The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Multiplexed Toxin Analysis Using Four Colors of Quantum Dot Fluororeagents
Abstract
Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe-ZnS core-shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassays for the detection of cholera toxin, ricin, shiga-like toxin 1, and staphylococcal enterotoxin B were performed simultaneously in single wells of a microtiter plate. Initially the assay performance for the detection of each toxin was examined. We then demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD-antibody reagents. Using a simple linear equation-based algorithm, it was possible to deconvolute the signal from mixed toxin samples, which allowed quantitation of all four toxins simultaneously.
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Dabbousi, B. O., Rodriguez-Viejo, J., Mikulec, F. V., Heine, J. R., Mattoussi, H., Ober, R., Jensen, K. F., & Bawendi, M. G. (1997) · The Journal of Physical Chemistry B · doi:10.1021/jp971091y · Cited by 4,229
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Gatchel, R. J., Peng, Y. B., Peters, M. L., Fuchs, P. N., & Turk, D. C. (2007) · Psychological Bulletin · doi:10.1037/0033-2909.133.4.581 · Cited by 3,392
Posthuma-Trumpie, G. A., Korf, J., & van Amerongen, A. (2009) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-008-2287-2 · Cited by 1,540
Freeman, R., Finder, T., & Willner, I. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200902395 · Cited by 490
Biosensors as useful tools for environmental analysis and monitoring
Rodriguez-Mozaz, S., Lopez de Alda, M. J., & Barceló, D. (2006) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-006-0574-3 · Cited by 451
Biosensing with Luminescent Semiconductor Quantum Dots
Sapsford, K. E., Pons, T., Medintz, I. L., & Mattoussi, H. (2006) · Sensors · doi:10.3390/s6080925 · Cited by 420
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Metadata
- Authors
- Goldman, E. R., Clapp, A. R., Anderson, G. P., Uyeda, H. T., Mauro, J. M., Medintz, I. L., & Mattoussi, H.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-12-20
- Volume
- 76
- Issue
- 3
- Pages
- 684-688
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2083835561
- Cited by
- 651
- References
- 1
- FWCI
- 18.65
- Type
- journal article
- Language
- en
- Open access
- closed
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