The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Micromechanical Detection of Proteins Using Aptamer-Based Receptor Molecules
Abstract
We report label-free protein detection using a microfabricated cantilever-based sensor that is functionalized with DNA aptamers to act as receptor molecules. The sensor utilizes two adjacent cantilevers that constitute a sensor/reference pair and allows direct detection of the differential bending between the two cantilevers. One cantilever is functionalized with aptamers selected for Taq DNA polymerase while the other is blocked with single-stranded DNA. We have found that the polymerase-aptamer binding induces a change in surface stress, which causes a differential cantilever bending that ranges from 3 to 32 nm depending on the ligand concentration. Protein recognition on the sensor surface is specific and has a concentration dependence that is similar to that in solution.
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Cites
Senturia, S. D. (2001) · Springer eBooks · doi:10.1007/b117574 · Cited by 2,072
Translating Biomolecular Recognition into Nanomechanics
Fritz, J., Baller, M. K., Lang, H. P., Rothuizen, H., Vettiger, P., Meyer, E., -J. Güntherodt, H., Gerber, C., & Gimzewski, J. K. (2000) · Science · doi:10.1126/science.288.5464.316 · Cited by 1,646
A femtojoule calorimeter using micromechanical sensors
Barnes, J. R., Stephenson, R. J., Woodburn, C. N., O’Shea, S. J., Welland, M. E., Rayment, T., Gimzewski, J. K., & Gerber, C. (1994) · Review of Scientific Instruments · doi:10.1063/1.1144509 · Cited by 270
Aptamer-based biosensor arrays for detection and quantification of biological macromolecules
McCauley, T. G., Hamaguchi, N., & Stanton, M. (2003) · Analytical Biochemistry · doi:10.1016/s0003-2697(03)00297-5 · Cited by 266
In vitro selection of signaling aptamers
Jhaveri, S., Rajendran, M., & Ellington, A. D. (2000) · Nature Biotechnology · doi:10.1038/82414 · Cited by 240
Self-citationAutomated selection of aptamers against protein targets translated in vitro: from gene to aptamer
Cox, J. C. (2002) · Nucleic Acids Research · doi:10.1093/nar/gnf107 · Cited by 173
Microfabricated mechanical biosensor with inherently differential readout
Savran, C. A., Burg, T. P., Fritz, J., & Manalis, S. R. (2003) · Applied Physics Letters · doi:10.1063/1.1605238 · Cited by 66
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Cited by
Analytical applications of aptamers
Tombelli, S., Minunni, M., & Mascini, M. (2005) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2004.11.006 · Cited by 1,017
Applications of Aptamers as Sensors
Cho, E. J., Lee, J.-W., & Ellington, A. D. (2009) · Annual Review of Analytical Chemistry · doi:10.1146/annurev.anchem.1.031207.112851 · Cited by 788
Self-citationMicro- and nanomechanical sensors for environmental, chemical, and biological detection
Waggoner, P. S., & Craighead, H. G. (2007) · Lab on a Chip · doi:10.1039/b707401h · Cited by 678
Bonora, M., Patergnani, S., Rimessi, A., De Marchi, E., Suski, J. M., Bononi, A., Giorgi, C., Marchi, S., Missiroli, S., Poletti, F., et al. (2012) · Purinergic Signalling · doi:10.1007/s11302-012-9305-8 · Cited by 644
Xiao, Y., Piorek, B. D., Plaxco, K. W., & Heeger, A. J. (2005) · Journal of the American Chemical Society · doi:10.1021/ja056555h · Cited by 527
Metadata
- Authors
- Savran, C. A., Knudsen, S. M., Ellington, A. D., & Manalis, S. R.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-04-29
- Volume
- 76
- Issue
- 11
- Pages
- 3194-3198
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2014100786
- Cited by
- 310
- References
- 7
- FWCI
- 8.64
- Type
- journal article
- Language
- en
- Open access
- closed
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