The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Single-Molecule Detection and Identification of Multiple Species by Multiparameter Fluorescence Detection
Abstract
Two general strategies are introduced to identify and quantify single molecules in dilute solutions by employing a spectroscopic method for data registration and specific burst analysis, denoted multiparameter fluorescence detection (MFD). MFD uses pulsed excitation and time-correlated single-photon counting to simultaneously monitor the evolution of the eight-dimensional fluorescence information (fundamental anisotropy, fluorescence lifetime, fluorescence intensity, time, excitation spectrum, fluorescence spectrum, fluorescence quantum yield, distance between fluorophores) in real time and allows for selection of specific events for subsequent analysis. Using the multiple fluorescence dimensions, we demonstrate a dye labeling scheme of oligonucleotides, by which it is possible to identify and separate 16 different compounds in the mixture via their characteristic pattern by MFD. Such identification procedures and multiplex assays with single-molecule sensitivity may have a great impact on screening of species and events that do not lend themselves so easily to amplification, such as disease-specific proteins and their interactions.
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Cites
Margittai, M., Widengren, J., Schweinberger, E., Schröder, G. F., Felekyan, S., Haustein, E., König, M., Fasshauer, D., Grubmüller, H., Jahn, R., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2331232100 · Cited by 290
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Cited by
Fluorescence lifetime imaging – techniques and applications
BECKER, W. (2012) · Journal of Microscopy · doi:10.1111/j.1365-2818.2012.03618.x · Cited by 890
Phase Transitions of Associative Biomacromolecules
Pappu, R. V., Cohen, S. R., Dar, F., Farag, M., & Kar, M. (2023) · Chemical Reviews · doi:10.1021/acs.chemrev.2c00814 · Cited by 367
Stimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
Self-citationKar, M., Dar, F., Welsh, T. J., Vogel, L. T., Kühnemuth, R., Majumdar, A., Krainer, G., Franzmann, T. M., Alberti, S., Seidel, C. A. M., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2202222119 · Cited by 270
Self-citationAccurate Single-Molecule FRET Studies Using Multiparameter Fluorescence Detection
Sisamakis, E., Valeri, A., Kalinin, S., Rothwell, P. J., & Seidel, C. A. M. (2010) · Methods in Enzymology · doi:10.1016/s0076-6879(10)75018-7 · Cited by 268
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Metadata
- Authors
- Widengren, J., Kudryavtsev, V., Antonik, M., Berger, S., Gerken, M., & Seidel, C. A. M.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-02-07
- Volume
- 78
- Issue
- 6
- Pages
- 2039-2050
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2050538415
- Cited by
- 217
- References
- 1
- FWCI
- 7.84
- Type
- journal article
- Language
- en
- Open access
- closed
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- Widengren, J., Kudryavtsev, V., Antonik, M., Berger, S., Gerken, M., & Seidel, C. A. M. (2006). Single-Molecule Detection and Identification of Multiple Species by Multiparameter Fluorescence Detection. Analytical Chemistry. https://doi.org/10.1021/ac0522759
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