The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Two-Dimensional Paper Network Format That Enables Simple Multistep Assays for Use in Low-Resource Settings in the Context of Malaria Antigen Detection
Open accessAbstract
The lateral flow test has become the standard bioassay format in low-resource settings because it is rapid, easy to use, and low in cost, uses reagents stored in dry form, and is equipment-free. However, lateral flow tests are often limited to a single chemical delivery step and not capable of the multistep processing characteristic of high performance laboratory-based assays. To address this limitation, we are developing a paper network platform that extends the conventional lateral flow test to two dimensions; this allows incorporation of multistep chemical processing, while still retaining the advantages of conventional lateral flow tests. Here, we demonstrate this format for an easy-to-use, signal-amplified sandwich format immunoassay for the malaria protein PfHRP2. The card contains reagents stored in dry form such that the user need only add sample and water. The multiple flows in the device are activated in a single user step of folding the card closed; the configuration of the paper network automatically delivers the appropriate volumes of (i) sample plus antibody conjugated to a gold particle label, (ii) a rinse buffer, and (iii) a signal amplification reagent to the capture region. These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Patterned Paper as a Platform for Inexpensive, Low‐Volume, Portable Bioassays
Martinez, A. W., Phillips, S. T., Butte, M. J., & Whitesides, G. M. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200603817 · Cited by 2,874
Posthuma-Trumpie, G. A., Korf, J., & van Amerongen, A. (2009) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-008-2287-2 · Cited by 1,540
Three-dimensional microfluidic devices fabricated in layered paper and tape
Martinez, A. W., Phillips, S. T., & Whitesides, G. M. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0810903105 · Cited by 1,246
Rapid tests for sexually transmitted infections (STIs): the way forward
Peeling, R. W., Holmes, K. K., Mabey, D., & Ronald, A. (2006) · Sexually Transmitted Infections · doi:10.1136/sti.2006.024265 · Cited by 599
Use of multiple colorimetric indicators for paper-based microfluidic devices
Dungchai, W., Chailapakul, O., & Henry, C. S. (2010) · Analytica Chimica Acta · doi:10.1016/j.aca.2010.06.019 · Cited by 364
Controlled reagent transport in disposable 2D paper networks
Fu, E., Lutz, B., Kauffman, P., & Yager, P. (2010) · Lab on a Chip · doi:10.1039/b919614e · Cited by 363
Self-citationMicrofluidics without pumps: reinventing the T-sensor and H-filter in paper networks
Osborn, J. L., Lutz, B., Fu, E., Kauffman, P., Stevens, D. Y., & Yager, P. (2010) · Lab on a Chip · doi:10.1039/c004821f · Cited by 338
Self-citationTransport in two-dimensional paper networks
Fu, E., Ramsey, S. A., Kauffman, P., Lutz, B., & Yager, P. (2011) · Microfluidics and Nanofluidics · doi:10.1007/s10404-010-0643-y · Cited by 313
Self-citationInkjet-printed paperfluidic immuno-chemical sensing device
Abe, K., Kotera, K., Suzuki, K., & Citterio, D. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4011-2 · Cited by 251
Quantitative biomarker assay with microfluidic paper-based analytical devices
Xu, L., Tian, J., & Shen, W. (2009) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-009-3195-9 · Cited by 135
Cited by
The present and future role of microfluidics in biomedical research
Sackmann, E. K., Fulton, A. L., & Beebe, D. J. (2014) · Nature · doi:10.1038/nature13118 · Cited by 2,995
Advances in paper-based point-of-care diagnostics
Hu, J., Wang, S., Wang, L., Li, F., Pingguan-Murphy, B., Lu, T. J., & Xu, F. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2013.10.075 · Cited by 956
Microfluidic Paper-Based Analytical Devices: From Design to Applications
Noviana, E., Ozer, T., Carrell, C. S., Link, J. S., McMahon, C., Jang, I., & Henry, C. S. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01335 · Cited by 631
Turning the Page: Advancing Paper-Based Microfluidics for Broad Diagnostic Application
Gong, M. M., & Sinton, D. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00024 · Cited by 573
Metadata
- Authors
- Fu, E., Liang, T., Spicar-Mihalic, P., Houghtaling, J., Ramachandran, S., & Yager, P.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-04-26
- Volume
- 84
- Issue
- 10
- Pages
- 4574-4579
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2023672403
- Cited by
- 272
- References
- 12
- FWCI
- 13.60
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Two-Dimensional Paper Network Format That Enables Simple Multistep Assays for Use in Low-Resource Settings in the Context of Malaria Antigen Detection. https://app.cochat.ai/papers/10.1021/ac300689s
- The paper
- Fu, E., Liang, T., Spicar-Mihalic, P., Houghtaling, J., Ramachandran, S., & Yager, P. (2012). Two-Dimensional Paper Network Format That Enables Simple Multistep Assays for Use in Low-Resource Settings in the Context of Malaria Antigen Detection. Analytical Chemistry. https://doi.org/10.1021/ac300689s
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
