The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Selection of DNA Aptamers against Epithelial Cell Adhesion Molecule for Cancer Cell Imaging and Circulating Tumor Cell Capture
Abstract
Epithelial cell adhesion molecule (EpCAM) is overexpressed in most solid cancers and is an ideal antigen for clinical applications in cancer diagnosis, prognosis, imaging, and therapy. Currently, most of the EpCAM-based diagnostic, prognostic, and therapeutic strategies rely on the anti-EpCAM antibody. However, the use of EpCAM antibody is restricted due to its large size and instability. In this study, we have successfully identified DNA aptamers that selectively bind human recombinant EpCAM protein. The aptamers can specifically recognize a number of live human cancer cells derived from breast, colorectal, and gastric cancers that express EpCAM but not bind to EpCAM-negative cells. Among the aptamer sequences identified, a hairpin-structured sequence SYL3 was optimized in length, resulting in aptamer sequence SYL3C. The Kd values of the SYL3C aptamer against breast cancer cell line MDA-MB-231 and gastric cancer cell line Kato III were found to be 38 ± 9 and 67 ± 8 nM, respectively, which are better than that of the full-length SYL3 aptamer. Flow cytometry analysis results indicated that the SYL3C aptamer was able to recognize target cancer cells from mixed cells in cell media. When used to capture cancer cells, up to 63% cancer cell capture efficiency was achieved with about 80% purity. With the advantages of small size, easy synthesis, good stability, high binding affinity, and selectivity, the DNA aptamers reported here against cancer biomarker EpCAM will facilitate the development of novel targeted cancer therapy, cancer cell imaging, and circulating tumor cell detection.
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Cites
Microchip-based immunomagnetic detection of circulating tumor cells
Hoshino, K., Huang, Y.-Y., Lane, N., Huebschman, M., Uhr, J. W., Frenkel, E. P., & Zhang, X. (2011) · Lab on a Chip · doi:10.1039/c1lc20270g · Cited by 400
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Aptamer-based targeted therapy
Zhu, G., & Chen, X. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.08.005 · Cited by 522
Liu, C., Zhao, J., Tian, F., Cai, L., Zhang, W., Feng, Q., Chang, J., Wan, F., Yang, Y., Dai, B., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-018-0343-6 · Cited by 482
Self-citationDiscovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein
Song, Y., Song, J., Wei, X., Huang, M., Sun, M., Zhu, L., Lin, B., Shen, H., Zhu, Z., & Yang, C. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c01394 · Cited by 451
Self-citationSong, Y., Shi, Y., Huang, M., Wang, W., Wang, Y., Cheng, J., Lei, Z., Zhu, Z., & Yang, C. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201809337 · Cited by 266
Self-citationSong, P., Ye, D., Zuo, X., Li, J., Wang, J., Liu, H., Hwang, M. T., Chao, J., Su, S., Wang, L., et al. (2017) · Nano Letters · doi:10.1021/acs.nanolett.7b01006 · Cited by 258
Metadata
- Authors
- Song, Y., Zhu, Z., An, Y., Zhang, W., Zhang, H., Liu, D., Yu, C., Duan, W., & Yang, C. J.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-03-29
- Volume
- 85
- Issue
- 8
- Pages
- 4141-4149
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2053277504
- Cited by
- 525
- References
- 1
- FWCI
- 12.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Song, Y., Zhu, Z., An, Y., Zhang, W., Zhang, H., Liu, D., Yu, C., Duan, W., & Yang, C. J. (2013). Selection of DNA Aptamers against Epithelial Cell Adhesion Molecule for Cancer Cell Imaging and Circulating Tumor Cell Capture. Analytical Chemistry. https://doi.org/10.1021/ac400366b
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