Electrochemical Detection of Single Microbeads Manipulated by Optical Tweezers in the Vicinity of Ultramicroelectrodes
Abstract
Latex micrometric beads are manipulated by optical tweezers in the vicinity of an ultramicroelectrode (UME). They are optically trapped in solution and approached the electrode surface. After the electrochemical measurement, they are optically removed from the surface. The residence time of the particle on the electrode is thus controlled by the optical tweezers. The detection is based on diffusional hindrance by the insulating objects which alters the fluxes of the redox Ru(NH3)6(3+) species toward the UME and thus its mass-transfer limited current. We have optically deposited successively 1, 2, and 3 beads of 3-μm radius on the UME surface, and we have recorded the variations of the current depending on their landing locations that were optically controlled. Finally we decreased the current by partially blocking the electroactive surface with a six-bead assembly. The variation of the steady-state current and the approach curves allow for the indirect electrochemical localization of the bead in the vicinity of the UME, not only when the bead is in contact but also when it is levitated at distances lower than the UME radius. These experiments show that single particles or more complex structures may be manipulated in situ in a contactless mode near the UME surface. From comparison with simulations, the electrochemical detection affords an indirect localization of the object in the UME environment. The developed approach offers a potential application for interrogating the electrochemical activity of single cells and nanoparticles.
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“18 The researchers demonstrated that the steady-state current ( i ss ) changes its value when particles hinder J M not just in contact with the UME, but also during contactless passages at distances lower than the UME radius.”
BackgroundMethodInfluential
- Authors
- Suraniti, E., Kanoufi, F., Gosse, C., Zhao, X., Dimova, R., Pouligny, B., & Sojic, N.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Max Planck Society (DE)
- Univ. Bordeaux, ENSCBP, ISM, UMR CNRS 5255, 33607 Pessac, France
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-09-16
- Volume
- 85
- Issue
- 19
- Pages
- 8902-8909
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2129423421
- Cited by
- 14
- References
- 41
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2014: 1
- FWCI
- 0.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- HAL (Le Centre pour la Communication Scientifique Directe) (submittedVersion)
- Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Orbital Angular Momentum in Optics
- Keywords
- optical tweezers, ultramicroelectrodes, single-particle detection, latex beads, optical trapping, contactless manipulation
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