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Fluorescent Probe-Based Lateral Flow Assay for Multiplex Nucleic Acid Detection
Open accessAbstract
Here we report a rapid, low cost, and disposable dipstick-type DNA biosensor that enables multiplex detection in a single assay. The fluorescent probes labeled with different fluorophores were introduced into the lateral flow nucleic acid testing system. In combination with multiple immobilized probes arranged in an array formant on the membrane, a dual-color fluorescent lateral flow DNA biosensor was developed using a portable fluorescence reader. Up to 13 human papillomavirus types could be detected simultaneously by a single-step operation in less than 30 min after linear-after-the-exponential (LATE)-PCR. The sensitivity was determined to be 10-10(2) copies plasmid DNA/μL. The specificity study showed no cross-reactivity among the 31 different common HPV types. In the clinical validation, 95.3% overall agreement showed very good potential for this method in the clinical application when compared to a commercial kit.
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Cited by
Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis
Liu, Y., Zhan, L., Qin, Z., Sackrison, J., & Bischof, J. C. (2021) · ACS Nano · doi:10.1021/acsnano.0c10035 · Cited by 626
Self-citationApplication of semiconductor quantum dots in bioimaging and biosensing
Martynenko, I. V., Litvin, A. P., Purcell-Milton, F., Baranov, A. V., Fedorov, A. V., & Gun'ko, Y. K. (2017) · Journal of Materials Chemistry B · doi:10.1039/c7tb01425b · Cited by 382
Point of care testing: The impact of nanotechnology
Syedmoradi, L., Daneshpour, M., Alvandipour, M., Gomez, F. A., Hajghassem, H., & Omidfar, K. (2017) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2016.08.084 · Cited by 367
Point-of-Care Diagnostics in Low Resource Settings: Present Status and Future Role of Microfluidics
Sharma, S., Zapatero-Rodríguez, J., Estrela, P., & O'Kennedy, R. (2015) · Biosensors · doi:10.3390/bios5030577 · Cited by 349
Stress Biomarkers in Biological Fluids and Their Point-of-Use Detection
Steckl, A. J., & Ray, P. (2018) · ACS Sensors · doi:10.1021/acssensors.8b00726 · Cited by 319
Banerjee, R., & Jaiswal, A. (2018) · The Analyst · doi:10.1039/c8an00307f · Cited by 265
Metadata
- Authors
- Xu, Y., Liu, Y., Wu, Y., Xia, X., Liao, Y., & Li, Q.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-06-06
- Volume
- 86
- Issue
- 12
- Pages
- 5611-5614
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2015554178
- Cited by
- 171
- References
- 0
- FWCI
- 6.46
- Type
- journal article
- Language
- en
- Open access
- bronze
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
