Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 168

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Multiplex Detection of Protease Activity with Quantum Dot Nanosensors Prepared by Intein-Mediated Specific Bioconjugation

Open access
Xia, Zuyong, Xing, Yun, So, Min-Kyung, Koh, Ai Leen, Sinclair, Robert, Rao, Jianghong·2008·Analytical Chemistry
DOI 10.1021/ac801562f
Open this paper in CoChat — free account

Abstract

We report here a protease sensing nanoplatform based on semiconductor nanocrystals or quantum dots (QDs) and bioluminescence resonance energy transfer (QD-BRET) to detect the protease activity in complex biological samples. These nanosensors consist of bioluminescent proteins as the BRET donor, quantum dots as the BRET acceptor, and protease substrates sandwiched between the two as a sensing group. An intein-mediated conjugation strategy was developed for site-specific conjugation of proteins to QDs in preparing these QD nanosensors. In this traceless ligation, the intein itself is spliced out and excluded from the final conjugation product. With this method, we have synthesized a series of QD nanosensors for highly sensitive detection of an important class of protease matrix metalloproteinase (MMP) activity. We demonstrated that these nanosensors can detect the MMP activity in buffers and in mouse serum with the sensitivity to a few nanograms per milliliter and secreted proteases by tumor cells. The suitability of these nanosensors for a multiplex protease assay has also been shown.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1021/ac801562f

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Research works printed but not linked: 44

  1. Fingleton B., 2006, Front. Biosci., 11, 479, 10.2741/1811 · DOI · Paper page
  2. Brinckerhoff C. E., 2002, Nat. Rev. Mol. Cell. Biol., 3, 207, 10.1038/nrm763 · DOI · Paper page
  3. Bachmeier B. E., 2005, Expert Rev. Anticancer Ther., 5, 149, 10.1586/14737140.5.1.149 · DOI
  4. Overall C. M., 2006, Nat. Rev. Cancer, 6, 227, 10.1038/nrc1821 · DOI · Paper page
  5. Murphy G., 1994, J. Biol. Chem., 269, 6632, 10.1016/S0021-9258(17)37419-7 · DOI · Paper page
  6. Knight C. G., 1992, FEBS Lett., 296, 263, 10.1016/0014-5793(92)80300-6 · DOI · Paper page
  7. Pham W., 2004, Bioconjugate Chem., 15, 1403, 10.1021/bc049924s · DOI
  8. McIntyre J. O., 2004, Biochem. J., 377, 617, 10.1042/bj20030582 · DOI · Paper page
  9. Bremer C., 2001, Nat. Med., 7, 743, 10.1038/89126 · DOI · Paper page
  10. Zhao M., 2003, Angew. Chem., Int. Ed., 42, 1375, 10.1002/anie.200390352 · DOI · Paper page
  11. Harris T. J., 2006, Angew. Chem., Int. Ed., 45, 3161, 10.1002/anie.200600259 · DOI
  12. Lepage M., 2007, Mol. Imaging, 6, 393, 10.2310/7290.2007.00035 · doi:10.2310/7290.2007.00035
  13. Bruchez M., 1998, Science, 281, 2013, 10.1126/science.281.5385.2013 · DOI · Paper page
  14. Michalet X., 2005, Science, 307, 538, 10.1126/science.1104274 · DOI · Paper page
  15. Medintz I. L., 2005, Nat. Mater., 4, 435, 10.1038/nmat1390 · DOI · Paper page
  16. Katz E., 2004, Angew. Chem., Int. Ed., 43, 6042, 10.1002/anie.200400651 · DOI · Paper page
  17. Daniel M.-C., 2004, Chem. Rev., 104, 293, 10.1021/cr030698+ · DOI · Paper page
  18. Alivisatos P., 2004, Nat. Biotechnol., 22, 47, 10.1038/nbt927 · DOI · Paper page
  19. Medintz I. L., 2004, Proc. Natl. Acad. Sci. U.S.A., 101, 9612, 10.1073/pnas.0403343101 · DOI · Paper page
  20. Medinitz I. L., 2003, Nat. Mater., 2, 630, 10.1038/nmat961 · DOI · Paper page
  21. Somers R. C., 2007, Chem. Soc. Rev., 36, 579, 10.1039/b517613c · DOI · Paper page
  22. Gao X., 2005, Curr. Opin. Biotechnol., 16, 63, 10.1016/j.copbio.2004.11.003 · DOI · Paper page
  23. Rosenthal S. J., 2008, J. Am. Chem. Soc., 130, 5720, 10.1021/ja710870e · DOI · Paper page
  24. Suzuki M., 2008, J. Am. Chem. Soc., 130, 5720, 10.1021/ja710870e · DOI · Paper page
  25. Shi L., 2006, J. Am. Chem. Soc., 128, 10378, 10.1021/ja063509o · DOI · Paper page
  26. Kim Y.-P., 2008, Anal. Chem., 80, 4634, 10.1021/ac702416e · doi:10.1021/ac702416e
  27. Troy T., 2004, Mol. Imaging, 3, 9, 10.1162/153535004773861688 · DOI · Paper page
  28. Contag P. R., 1998, Nat. Med., 4, 245, 10.1038/nm0298-245 · DOI · Paper page
  29. Contag C. H., 2002, Annu. Rev. Biomed. Eng., 4, 35, 10.1146/annurev.bioeng.4.111901.093336 · DOI · Paper page
  30. So M.-K., 2006, Nat. Biotechnol., 24, 339, 10.1038/nbt1188 · DOI · Paper page
  31. So M.-K., 2006, Nat. Protocols, 1, 1160, 10.1038/nprot.2006.162 · DOI
  32. Evans T. C., 2002, Chem. Rev., 102, 4869, 10.1021/cr9601369 · DOI
  33. Muralidharan V., 2006, Nat. Methods, 3, 429, 10.1038/nmeth886 · DOI · Paper page
  34. Muir T. W., 2003, Annu. Rev. Biochem., 72, 249, 10.1146/annurev.biochem.72.121801.161900 · DOI · Paper page
  35. Liu X. Q., 2000, Annu. Rev. Genetics, 34, 61, 10.1146/annurev.genet.34.1.61 · DOI
  36. Loening A. M, 2006, Protein Eng., Des. Sel., 19, 391, 10.1093/protein/gzl023 · DOI · Paper page
  37. Turk B. E., 2001, Nat. Biotechnol., 19, 661, 10.1038/90273 · DOI · Paper page
  38. Telenti A., 1997, J. Bacteriol., 179, 6378, 10.1128/jb.179.20.6378-6382.1997 · doi:10.1128/jb.179.20.6378-6382.1997
  39. Kalia J., 2006, ChemBioChem, 7, 1375, 10.1002/cbic.200600150 · DOI
  40. Law B., 2004, Chem. Biol., 11, 99, 10.1016/j.chembiol.2003.12.017 · DOI
  41. Giambernardi T. A., 1998, Matrix Biol., 16, 483, 10.1016/S0945-053X(98)90019-1 · DOI · Paper page
  42. Stahtea X. N., 2007, Int. J. Cancer, 121, 2808, 10.1002/ijc.23029 · DOI
  43. Zhang Y., 2006, Nano Lett., 6, 1988, 10.1021/nl0611586 · DOI
  44. Zhang Y., 2006, Angew. Chem., Int. Ed., 45, 4936, 10.1002/anie.200601197 · DOI · Paper page

Cited by

See all 168

Metadata

Authors
Xia, Z., Xing, Y., So, M.-K., Koh, A. L., Sinclair, R., & Rao, J.
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2008-10-16
Volume
80
Issue
22
Pages
8649-8655
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/ac801562f
OpenAlex
W2015859382
Cited by
168
References
2
FWCI
7.58
Type
journal article
Language
en
Open access
green

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.