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Cited by 385

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Refinements to Label Free Proteome Quantitation: How to Deal with Peptides Shared by Multiple Proteins

Zhang, Ying, Wen, Zhihui, Washburn, Michael P., Florens, Laurence·2010·Analytical Chemistry
DOI 10.1021/ac9023999
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Abstract

Quantitative shotgun proteomics is dependent on the detection, identification, and quantitative analysis of peptides. An issue arises with peptides that are shared between multiple proteins. What protein did they originate from and how should these shared peptides be used in a quantitative proteomics workflow? To systematically evaluate shared peptides in label-free quantitative proteomics, we devised a well-defined protein sample consisting of known concentrations of six albumins from different species, which we added to a highly complex yeast lysate. We used the spectral counts based normalized spectral abundance factor (NSAF) as the starting point for our analysis and compared an exhaustive list of possible combinations of parameters to determine what was the optimal approach for dealing with shared peptides and shared spectral counts. We showed that distributing shared spectral counts based on the number of unique spectral counts led to the most accurate and reproducible results.

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Research works printed but not linked: 16

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Metadata

Authors
Zhang, Y., Wen, Z., Washburn, M. P., & Florens, L.
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2010-02-18
Volume
82
Issue
6
Pages
2272-2281
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/ac9023999
OpenAlex
W2078138443
Cited by
385
References
2
FWCI
13.96
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Refinements to Label Free Proteome Quantitation: How to Deal with Peptides Shared by Multiple Proteins. https://app.cochat.ai/papers/10.1021/ac9023999
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Zhang, Y., Wen, Z., Washburn, M. P., & Florens, L. (2010). Refinements to Label Free Proteome Quantitation: How to Deal with Peptides Shared by Multiple Proteins. Analytical Chemistry. https://doi.org/10.1021/ac9023999

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