The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Localization of O-Glycosylation Sites in Peptides by Electron Capture Dissociation in a Fourier Transform Mass Spectrometer
Abstract
The novel technique electron capture dissociation (ECD) of electrospray generated [M + nH]n+ polypeptide cations produces rapid cleavage of the backbone NH-Ca bond to form c and z ions (in the modified notation of Roepstorff and Fohlman). The potential of the Fourier transform mass spectrometry equipped with ECD in structure analysis of O-glycosylated peptides in the 3 kDa range has been investigated. Totally, 85% of the available interresidue bonds were cleaved in five glycopeptides; more stable c ions accounted for 62% of the observed fragmentation. The c series provided direct evidence on the glycosylation sites in every case studied, with no glycan (GalNAc and dimannose) losses observed from these species. Less stable z ions supported the glycan site assignment, with minor glycan detachments. These losses, as well as the observed formation of even-electron z ions, are attributed to radical-site-initiated reactions. In favorable cases, complete sequence and glycan position information is obtained from a single-scan spectrum. The "mild" character of ECD supports the previously proposed non-ergodic (cleavage prior to energy randomization) mechanism, and the low internal energy increment of fragments.
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Citations
Cites
Roepstorff, P., & Fohlman, J. (1984) · Biological Mass Spectrometry · doi:10.1002/bms.1200111109 · Cited by 2,633
Self-citationElectron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process
Zubarev, R. A., Kelleher, N. L., & McLafferty, F. W. (1998) · Journal of the American Chemical Society · doi:10.1021/ja973478k · Cited by 1,881
Self-citationInt. J. Mass Spectrom. Ion Processes
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Zubarev, R. A., Kruger, N. A., Fridriksson, E. K., Lewis, M. A., Horn, D. M., Carpenter, B. K., & McLafferty, F. W. (1999) · Journal of the American Chemical Society · doi:10.1021/ja981948k · Cited by 557
Self-citationKelleher, N. L., Zubarev, R. A., Bush, K., Furie, B., Furie, B. C., McLafferty, F. W., & Walsh, C. T. (1999) · Analytical Chemistry · doi:10.1021/ac990684x · Cited by 368
Self-citationTandem Mass Spectrometry of Large Biomolecule Ions by Blackbody Infrared Radiative Dissociation
Price, W. D., Schnier, P. D., & Williams, E. R. (1996) · Analytical Chemistry · doi:10.1021/ac951038a · Cited by 314
Wandall, H. H., Hassan, H., Mirgorodskaya, E., Kristensen, A. K., Roepstorff, P., Bennett, E. P., Nielsen, P. A., Hollingsworth, M. A., Burchell, J., Taylor-Papadimitriou, J., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.38.23503 · Cited by 303
Self-citationCharacterization of protein glycosylation by mass spectrometry
Burlingame, A. L. (1996) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(96)80088-7 · Cited by 65
Determination of the thermal energy and its distribution in peptides
Drahos, L., & Vékey, K. (1999) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(98)00156-1 · Cited by 45
Cited by
Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry
Syka, J. E. P., Coon, J. J., Schroeder, M. J., Shabanowitz, J., & Hunt, D. F. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402700101 · Cited by 2,370
Electron Capture Dissociation for Structural Characterization of Multiply Charged Protein Cations
Zubarev, R. A., Horn, D. M., Fridriksson, E. K., Kelleher, N. L., Kruger, N. A., Lewis, M. A., Carpenter, B. K., & McLafferty, F. W. (2000) · Analytical Chemistry · doi:10.1021/ac990811p · Cited by 986
Self-citationThe utility of ETD mass spectrometry in proteomic analysis
Mikesh, L. M., Ueberheide, B., Chi, A., Coon, J. J., Syka, J. E. P., Shabanowitz, J., & Hunt, D. F. (2006) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2006.10.003 · Cited by 535
Reactions of polypeptide ions with electrons in the gas phase
Zubarev, R. A. (2003) · Mass Spectrometry Reviews · doi:10.1002/mas.10042 · Cited by 431
Self-citationHigh-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
‘Top down’ protein characterization via tandem mass spectrometry
Reid, G. E., & McLuckey, S. A. (2002) · Journal of Mass Spectrometry · doi:10.1002/jms.346 · Cited by 285
Mucin‐type O‐glycosylation – putting the pieces together
Jensen, P. H., Kolarich, D., & Packer, N. H. (2010) · The FEBS Journal · doi:10.1111/j.1742-4658.2009.07429.x · Cited by 252
Ge, Y., Rybakova, I. N., Xu, Q., & Moss, R. L. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0813369106 · Cited by 154
Advances in mass spectrometry driven O-glycoproteomics
Levery, S. B., Steentoft, C., Halim, A., Narimatsu, Y., Clausen, H., & Vakhrushev, S. Y. (2015) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2014.09.026 · Cited by 111
Sidoli, S., Schwämmle, V., Ruminowicz, C., Hansen, T. A., Wu, X., Helin, K., & Jensen, O. N. (2014) · PROTEOMICS · doi:10.1002/pmic.201400084 · Cited by 87
Metadata
- Authors
- Mirgorodskaya, E., Roepstorff, P., & Zubarev, R. A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-09-15
- Volume
- 71
- Issue
- 20
- Pages
- 4431-4436
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2064140463
- Cited by
- 381
- References
- 9
- FWCI
- 9.38
- Type
- journal article
- Language
- en
- Open access
- closed
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