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Cited by 31Bacterial Identification and Susceptibility TestingMass Spectrometry Techniques and ApplicationsAntibiotic Resistance in Bacteria

Plasmonic Colloidosome-Coupled MALDI-TOF MS for Bacterial Heteroresistance Study at Single-Cell Level

Dai, Yuchen, Li, Chenyu, Yi, Jia, Qin, Qin, Liu, Baohong, Qiao, Liang·2020·Analytical Chemistry
DOI 10.1021/acs.analchem.0c00494
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Abstract

Antimicrobial resistance (AMR) is a long-term public health challenge worldwide, and it is increasingly recognized to be a heteroresistance phenomenon in an isogenic bacterial population. When the minority population of resistant bacteria with strong AMR is not handled in time, such a subpopulation can be enriched leading to the further development of bacterial AMR. However, conventional AMR studies based on ensemble-averaged data from a large population fail to characterize the bacterial heterogeneity. In this work, we develop a method using plasmonic colloidosomes and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to study single bacterial cell AMR. The plasmonic colloidosomes act simultaneously as bacteria containers and sample spots for MALDI-TOF MS detection. Cells of β-lactamase-producing Escherichia coli ( E. coli ) are trapped in colloidosome containers (∼200 μm in diameter) in the presence of antibiotic drug ampicillin (AMP). Benefiting from the fast reaction kinetics in microcompartments, the hydrolysis product of AMP by bacteria can be detected by MALDI-TOF MS within 40 min. The colloidosomes as MALDI sample spots also benefit sensitive detection and accurate quantification of AMP and its hydrolysis product. It was found that even an isogenic population could consist of a mixture of bacteria that have different resistance degrees to antibiotics. Taking the β-lactamase-producing E. coli as an example, 20% of the bacterial individuals have relatively strong activity in hydrolyzing AMP. It is expected that the colloidosome-based platform would reveal a prospective application in full characterization of single bacterial cell AMR.

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  • Single-cell assays, including flow cytometry, scRNA-seq, and microfluidics-based assays, allow analysis of gene expression, cell viability, and drug tolerance in individual fungal cells.

    “These include techniques such as flow cytometry, single-cell RNA sequencing (scRNA-seq), and microfluidics-based assays, allowing for the analysis of gene expression, cell viability, and drug tolerance in individual fungal cells [170,171].”

    From Insight into the Mechanisms and Clinical Relevance of Antifungal Heteroresistance (2025) · cites this paper as [171]

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Authors
Dai, Y., Li, C., Yi, J., Qin, Q., Liu, B., & Qiao, L.
Affiliations
  • Fudan University (CN)
  • Second Military Medical University (CN)
Corresponding author
Qiao, Liang
ORCID
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2020-05-04
Volume
92
Issue
12
Pages
8051-8057
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/acs.analchem.0c00494
OpenAlex
W3023353895
Cited by
31
References
32
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 6 · 2022: 11 · 2021: 5
FWCI
3.64
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Analytical Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Science and Technology Commission of Shanghai Municipality (17JC1401900, 18441901000)
  • National Natural Science Foundation of China (21934001, 81671849)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
Keywords
antimicrobial resistance, single-cell analysis, MALDI-TOF mass spectrometry, beta-lactamase, Escherichia coli, microcompartments, antibiotic detection
MeSH terms
Anti-Bacterial Agents, beta-Lactamases, Escherichia coli, Microbial Sensitivity Tests, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Drug Resistance, Bacterial, Single-Cell Analysis

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