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Chemoproteomic Approach toward Probing the Interactomes of Perfluoroalkyl Substances
Abstract
Poly- and perfluoroalkyl substances (PFASs) are widely used in industrial products and consumer goods. Due to their extremely recalcitrant nature and potential bioaccumulation and toxicity, exposure to PFASs may result in adverse health outcomes in humans and wildlife. In this study, we developed a chemoproteomic strategy, based on the use of isotope-coded desthiobiotin-perfluorooctanephosphonic acid (PFOPA) probe and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis, to profile PFAS-binding proteins. Targeted proteins were labeled with the desthiobiotin-PFOPA probe, digested with trypsin, and the ensuing desthiobiotin-conjugated peptides were enriched with streptavidin beads for LC-MS/MS analysis. We were able to identify 469 putative PFOPA-binding proteins. By conducting competitive binding experiments using low (10 μM) and high (100 μM) concentrations of stable isotope-labeled PFOPA probes, we further identified 128 nonredundant peptides derived from 75 unique proteins that exhibit selective binding toward PFOPA. Additionally, we demonstrated that one of these proteins, fatty acid-binding protein 5 (FABP5), could interact directly with PFASs, including perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), perfluorohexanesulfonic acid (PFHxS), and perfluorobutanesulfonic acid (PFBS). Furthermore, desthiobiotin-labeled lysine residues are located close to the fatty acid-binding pocket of FABP5, and the binding affinity varies with the structures of PFASs. Taken together, we developed a novel chemoproteomic method for interrogating the PFAS-interacting proteome. The identification of these proteins sets the stage for understanding the mechanisms through which exposure to PFASs confers adverse human health effects.
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“Indeed, a recent study has analyzed the human protein ‘interactome’ of perfluorooctanephosphonic acid (PFOPA) and identified 469 PFOPA‐binding proteins (Zhang et al., 2021).”
From Nothing lasts forever: understanding microbial biodegradation of polyfluorinated compounds and perfluorinated alkyl substances (2022) · cites this paper as Zhang et al., 2021
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Perez-Riverol, Y., Csordas, A., Bai, J., Bernal-Llinares, M., Hewapathirana, S., Kundu, D. J., Inuganti, A., Griss, J., Mayer, G., Eisenacher, M., et al. (2019) · Nucleic Acids Research · doi:10.1093/nar/gky1106 · Cited by 6,693
A subcellular map of the human proteome
Thul, P. J., Åkesson, L., Wiking, M., Mahdessian, D., Geladaki, A., Ait Blal, H., Alm, T., Asplund, A., Björk, L., Breckels, L. M., et al. (2017) · Science · doi:10.1126/science.aal3321 · Cited by 2,813
A Never-Ending Story of Per- and Polyfluoroalkyl Substances (PFASs)?
Wang, Z., DeWitt, J. C., Higgins, C. P., & Cousins, I. T. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.6b04806 · Cited by 1,921
Quantitative reactivity profiling predicts functional cysteines in proteomes
Weerapana, E., Wang, C., Simon, G. M., Richter, F., Khare, S., Dillon, M. B. D., Bachovchin, D. A., Mowen, K., Baker, D., & Cravatt, B. F. (2010) · Nature · doi:10.1038/nature09472 · Cited by 1,684
Conder, J. M., Hoke, R. A., Wolf, W. D., Russell, M. H., & Buck, R. C. (2008) · Environmental Science & Technology · doi:10.1021/es070895g · Cited by 1,206
Wang, Z., Cousins, I. T., Scheringer, M., & Hungerbühler, K. (2013) · Environment International · doi:10.1016/j.envint.2013.08.021 · Cited by 924
Geiger, T., Wehner, A., Schaab, C., Cox, J., & Mann, M. (2012) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m111.014050 · Cited by 797
Sun, M., Arevalo, E., Strynar, M., Lindstrom, A., Richardson, M., Kearns, B., Pickett, A., Smith, C., & Knappe, D. R. U. (2016) · Environmental Science & Technology Letters · doi:10.1021/acs.estlett.6b00398 · Cited by 688
Wang, Z., Cousins, I. T., Scheringer, M., & Hungerbuehler, K. (2015) · Environment International · doi:10.1016/j.envint.2014.11.013 · Cited by 627
Global profiling of lysine reactivity and ligandability in the human proteome
Hacker, S. M., Backus, K. M., Lazear, M. R., Forli, S., Correia, B. E., & Cravatt, B. F. (2017) · Nature Chemistry · doi:10.1038/nchem.2826 · Cited by 485
Comparing the toxic potency in vivo of long-chain perfluoroalkyl acids and fluorinated alternatives
Gomis, M. I., Vestergren, R., Borg, D., & Cousins, I. T. (2018) · Environment International · doi:10.1016/j.envint.2018.01.011 · Cited by 438
Field, C. S., Baixauli, F., Kyle, R. L., Puleston, D. J., Cameron, A. M., Sanin, D. E., Hippen, K. L., Loschi, M., Thangavelu, G., Corrado, M., et al. (2020) · Cell Metabolism · doi:10.1016/j.cmet.2019.11.021 · Cited by 425
A road map to evaluate the proteome-wide selectivity of covalent kinase inhibitors
Lanning, B. R., Whitby, L. R., Dix, M. M., Douhan, J., Gilbert, A. M., Hett, E. C., Johnson, T., Joslyn, C. M., Kath, J. C., Niessen, S., et al. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1582 · Cited by 364
Xu, Y., Fletcher, T., Pineda, D., Lindh, C. H., Nilsson, C., Glynn, A., Vogs, C., Norström, K., Lilja, K., Jakobsson, K., et al. (2020) · Environmental Health Perspectives · doi:10.1289/ehp6785 · Cited by 342
Eschauzier, C., Beerendonk, E., Scholte-Veenendaal, P., & De Voogt, P. (2012) · Environmental Science & Technology · doi:10.1021/es201662b · Cited by 318
Sheng, N., Cui, R., Wang, J., Guo, Y., Wang, J., & Dai, J. (2018) · Archives of Toxicology · doi:10.1007/s00204-017-2055-1 · Cited by 295
Zhang, L., Ren, X.-M., & Guo, L.-H. (2013) · Environmental Science & Technology · doi:10.1021/es4026722 · Cited by 267
Ecotoxicological Evaluation of Perfluorooctanesulfonate (PFOS)
Beach, S. A., Newsted, J. L., Coady, K. K., & Giesy, J. P. (2006) · Reviews of Environmental Contamination and Toxicology · doi:10.1007/0-387-32883-1_5 · Cited by 233
Klemes, M. J., Ling, Y., Ching, C., Wu, C., Xiao, L., Helbling, D. E., & Dichtel, W. R. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201905142 · Cited by 185
Cyclodextrin Polymers with Nitrogen-Containing Tripodal Crosslinkers for Efficient PFAS Adsorption
Yang, A., Ching, C., Easler, M., Helbling, D. E., & Dichtel, W. R. (2020) · ACS Materials Letters · doi:10.1021/acsmaterialslett.0c00240 · Cited by 151
Nian, M., Luo, K., Luo, F., Aimuzi, R., Huo, X., Chen, Q., Tian, Y., & Zhang, J. (2020) · Environmental Science & Technology · doi:10.1021/acs.est.0c02444 · Cited by 148
Cheng, W., & Ng, C. A. (2018) · Environmental Science & Technology · doi:10.1021/acs.est.8b01268 · Cited by 141
Armstrong, E. H., Goswami, D., Griffin, P. R., Noy, N., & Ortlund, E. A. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.514646 · Cited by 140
Rao, U., Su, Y., Khor, C. M., Jung, B., Ma, S., Cwiertny, D. M., Wong, B. M., & Jassby, D. (2020) · Environmental Science & Technology · doi:10.1021/acs.est.0c02773 · Cited by 117
Interaction of perfluoroalkyl acids with human liver fatty acid-binding protein
Sheng, N., Li, J., Liu, H., Zhang, A., & Dai, J. (2014) · Archives of Toxicology · doi:10.1007/s00204-014-1391-7 · Cited by 113
Highly Selective Removal of Perfluorinated Contaminants by Adsorption on All‐Silica Zeolite Beta
den Bergh, M. V., Krajnc, A., Voorspoels, S., Tavares, S. R., Mullens, S., Beurroies, I., Maurin, G., Mali, G., & Vos, D. E. D. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.202002953 · Cited by 110
Cao, H., Zhou, Z., Wang, L., Liu, G., Sun, Y., Wang, Y., Wang, T., & Liang, Y. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.8b05564 · Cited by 92
Self-citationYang, C., Lee, H. K., Zhang, Y., Jiang, L.-L., Chen, Z., Chung, A. C. K., & Cai, Z. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b00711 · Cited by 70
Shao, X., Ji, F., Wang, Y., Zhu, L., Zhang, Z., Du, X., Chung, A. C. K., Hong, Y., Zhao, Q. C., & Cai, Z. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b02995 · Cited by 48
Self-citationMiao, W., Li, L., & Wang, Y. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b00986 · Cited by 37
Self-citationQiu, H., & Wang, Y. (2007) · Analytical Chemistry · doi:10.1021/ac0622375 · Cited by 33
Self-citationHuang, Q., Zhang, J., Peng, S., Du, M., Ow, S. Y., Pu, H., Pan, C., & Shen, H. (2013) · Journal of Applied Toxicology · doi:10.1002/jat.2963 · Cited by 23
Miao, W., Xiao, Y., Guo, L., Jiang, X., Huang, M., & Wang, Y. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b02816 · Cited by 23
Self-citationCai, R., Huang, M., & Wang, Y. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b03727 · Cited by 15
Self-citationGuo, L., Xiao, Y., & Wang, Y. (2014) · Analytical Chemistry · doi:10.1021/ac502592d · Cited by 12
Self-citationXiao, Y., Ji, D., Guo, L., & Wang, Y. (2014) · Analytical Chemistry · doi:10.1021/ac500588q · Cited by 11
Self-citationPFASs, sex hormones and asthma
Greenhill, C. (2017) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2017.58 · Cited by 11
Chemical Proteomic Profiling of Lysophosphatidic Acid-Binding Proteins
Dong, X., Gao, L., Song, J., & Wang, Y. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b04850 · Cited by 9
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Cited by
Wackett, L. P. (2022) · Microbial Biotechnology · doi:10.1111/1751-7915.13928 · Cited by 165
Metadata
- Authors
- Zhang, Q., Dong, X., Lu, J., Song, J., & Wang, Y.
- ORCID
- Wang, Yinsheng
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2021-06-29
- Volume
- 93
- Issue
- 27
- Pages
- 9634-9639
- ISSN
- 0003-2700
- 1520-6882
- PMC
- PMC8760635
- Cited by
- 14
- References
- 38
- Language
- en
- Licence
- Funders
- National Institute of Environmental Health Sciences (R35 ES031707)
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