Differences in the Elemental Isotope Definition May Lead to Errors in Modern Mass-Spectrometry-Based Proteomics
Abstract
The elemental isotope definition used to calculate the theoretical masses and isotope distribution of (bio)molecules is considered to be a fixed, universal standard in mass-spectrometry-based proteomics. However, this is an incorrect assumption. In view of the ongoing advances in mass spectrometry technology, and in particular the ever-increasing mass precision, the elemental isotope definition and its variations should be taken into account. We illustrate the effect of the elemental isotope uncertainty on the theoretical and experimental masses with theoretical calculations and examples.
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Mass measurement alone of intact proteins and complexes is limited because their average masses might shift slightly due to natural variations in isotopic abundance, causing mass shifts greater than the accuracy of modern high-end mass spectrometers.
“Another limitation of mass measurement alone of intact proteins and complexes is that their (average) masses might shift slightly due to natural variations in isotopic abundance, an effect which is able to cause mass shifts greater than the accuracy of modern high-end mass spectrometers.”
From Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry (2020) · cites this paper as [51]
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Self-citation
- Authors
- Claesen, J., Lermyte, F., Sobott, F., Burzykowski, T., & Valkenborg, D.
- Affiliations
- Hasselt University (BE)
- University of Antwerp (BE)
- Flemish Institute for Technological Research (BE)
- Corresponding author
- Claesen, Jürgen
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-10-22
- Volume
- 87
- Issue
- 21
- Pages
- 10747-10754
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2416554609
- Cited by
- 9
- References
- 28
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Document Server@UHasselt (UHasselt) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- isotope distribution, mass spectrometry-based proteomics
- MeSH terms
- Animals, Apoproteins, Elements, Horses, Isotope Labeling, Isotopes, Molecular Weight, Myoglobin, Mass Spectrometry, Proteomics
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