The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Bioresponsive Release System for Visual Fluorescence Detection of Carcinoembryonic Antigen from Mesoporous Silica Nanocontainers Mediated Optical Color on Quantum Dot-Enzyme-Impregnated Paper
Abstract
An all-in-one paper-based analytical device (PAD) was successfully developed for visual fluorescence detection of carcinoembryonic antigen (CEA) on CdTe/CdSe quantum dot (QD)-enzyme-impregnated paper by coupling with a bioresponsive controlled-release system from DNA-gated mesoporous silica nanocontainers (MSNs). The assay was carried out in a centrifuge tube by using glucose-loaded MSNs with a CEA aptamer and a QD-enzyme-paper attached on the lid. Initially, single-strand complementary DNA to a CEA aptamer was covalently conjugated to the aminated MSN, and then glucose (enzyme substrate) molecules were gated into the pore with the help of the aptamer. Glucose oxidase (GOD) and CdTe/CdSe QDs were coimmobilized on paper for the visual fluorescence signal output. Upon target CEA introduction in the detection cell, the analyte specifically reacted with the immobilized aptamer on the MSN to open the pore, thereby resulting in the glucose release. The released glucose was oxidized by the immobilized GOD on paper to produce gluconic acid and hydrogen peroxide, and the latter quenched the fluorescence of CdTe/CdSe QDs, which could be determined by the naked eye on a portable smartphone and a commercial fluorospectrometer. Under optimal conditions, the PAD-based sensing system enabled sensitive discrimination of target CEA against other biomarkers or proteins in a linear range of 0.05–20 ng mL –1 with a limit of detection of 6.7 pg mL –1 (ppt). In addition, our strategy displayed high specificity, good reproducibility, and acceptable accuracy for analyzing human serum specimens with a commercial human CEA ELISA kit. Importantly, this methodology offers promise for simple analysis of biological samples and is suitable for use in the mass production of miniaturized devices, thus opening new opportunities for protein diagnostics and biosecurity.
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Citations
Cites
Mesoporous silica nanoparticles for drug delivery and biosensing applications
Slowing, I. I., Trewyn, B. G., Giri, S., & Lin, V. S. ‐. (2007) · Advanced Functional Materials · doi:10.1002/adfm.200601191 · Cited by 1,699
Cited by
Recent advances in isolation and detection of exosomal microRNAs related to Alzheimer's disease
Pishbin, E., Sadri, F., Dehghan, A., Kiani, M. J., Hashemi, N., Zare, I., Mousavi, P., & Rahi, A. (2023) · Environmental Research · doi:10.1016/j.envres.2023.115705 · Cited by 25
Metadata
- Authors
- Qiu, Z., Shu, J., & Tang, D.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-04-12
- Volume
- 89
- Issue
- 9
- Pages
- 5152-5160
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2604463251
- Cited by
- 567
- References
- 1
- FWCI
- 22.81
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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