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Cited by 257

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System

Open access
Yuan, Chaoqun, Tian, Tian, Sun, Jian, Hu, Menglu, Wang, Xusheng, Xiong, Erhu, Cheng, Meng, Bao, Yijuan, Lin, Wei, Jiang, Jieming, Yang, Chengwei, Chen, Qian, Zhang, Huang, Wang, Heng, Wang, Xiran, Deng, Xianbo, Liao, Xiaoping, Liu, Yahong, Wang, Zhang, Zhang, Guihong, Zhou, Xiaoming·2020·Analytical Chemistry
DOI 10.1021/acs.analchem.9b05597
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Abstract

Abstract Colorimetric gene detection based on gold nanoparticles (AuNPs) is an attractive detection format due to its simplicity. Here, we report a new design for a colorimetric gene-sensing platform based on the CRISPR/Cas system that has improved specificity, sensitivity, and universality. CRISPR/Cas12a and CRISPR/Cas13a have two distinct catalytic activities and are used for specific target gene recognition. Programmable recognition of DNA by Cas12a/crRNA and RNA by Cas13a/crRNA with a complementary sequence activates the nonspecific trans -ssDNA or -RNA cleavage, respectively, thus degrading the ssDNA or RNA linkers which are designed as a hybridization template for the AuNP-DNA probe pair. Target-induced trans -ssDNA or RNA cleavage leads to a distance-dependent color change for the AuNP-DNA probe pair. In this platform, naked eye detection of transgenic rice, African swine fever virus (ASFV), and a miRNA can be completed within 1 hour. Our colorimetric gene-sensing method shows superior characteristics, such as probe universality, isothermal reaction conditions, on-site detection capability, and sensitivity that is comparable to that of the fluorescent detection; thus, this method represents a robust next generation gene detection platform.

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Metadata

Authors
Yuan, C., Tian, T., Sun, J., Hu, M., Wang, X., Xiong, E., Cheng, M., Bao, Y., Lin, W., Jiang, J., Yang, C., Chen, Q., Zhang, H., Wang, H., Wang, X., Deng, X., Liao, X., Liu, Y., Wang, Z., Zhang, G., & Zhou, X.
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2020-02-07
Volume
92
Issue
5
Pages
4029-4037
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/acs.analchem.9b05597
OpenAlex
W3004469303
Cited by
257
References
45
FWCI
11.82
Type
journal article
Language
en
Open access
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CoChat. (2026). Citation check: Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System. https://app.cochat.ai/papers/10.1021/acs.analchem.9b05597
The paper
Yuan, C., Tian, T., Sun, J., Hu, M., Wang, X., Xiong, E., Cheng, M., Bao, Y., Lin, W., Jiang, J., Yang, C., Chen, Q., Zhang, H., Wang, H., Wang, X., Deng, X., Liao, X., Liu, Y., Wang, Z., . . . Zhou, X. (2020). Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.9b05597

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.