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Cited by 124Gold and Silver Nanoparticles Synthesis and ApplicationsSpectroscopy Techniques in Biomedical and Chemical ResearchAnalytical chemistry methods development

Surface Enhanced Raman Spectroscopy for Quantitative Analysis: Results of a Large-Scale European Multi-Instrument Interlaboratory Study

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Fornasaro, Stefano, Alsamad, Fatima, Baia, Monica, Batista de Carvalho, Luís A. E., Beleites, Claudia, Byrne, Hugh J., Chiadò, Alessandro, Chis, Mihaela +36 more·2020·Analytical Chemistry
DOI 10.1021/acs.analchem.9b05658PMC PMC7997108
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Abstract

Surface-enhanced Raman scattering (SERS) is a powerful and sensitive technique for the detection of fingerprint signals of molecules and for the investigation of a series of surface chemical reactions. Many studies introduced quantitative applications of SERS in various fields, and several SERS methods have been implemented for each specific application, ranging in performance characteristics, analytes used, instruments, and analytical matrices. In general, very few methods have been validated according to international guidelines. As a consequence, the application of SERS in highly regulated environments is still considered risky, and the perception of a poorly reproducible and insufficiently robust analytical technique has persistently retarded its routine implementation. Collaborative trials are a type of interlaboratory study (ILS) frequently performed to ascertain the quality of a single analytical method. The idea of an ILS of quantification with SERS arose within the framework of Working Group 1 (WG1) of the EU COST Action BM1401 Raman4Clinics in an effort to overcome the problematic perception of quantitative SERS methods. Here, we report the first interlaboratory SERS study ever conducted, involving 15 laboratories and 44 researchers. In this study, we tried to define a methodology to assess the reproducibility and trueness of a quantitative SERS method and to compare different methods. In our opinion, this is a first important step toward a "standardization" process of SERS protocols, not proposed by a single laboratory but by a larger community.

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  • NPs synthesized by LAL are of particular interest because their clean surface allows functional molecules to reach easily the NP surface.

    “NPs synthesized by LAL are of particular interest because of their clean surface, which allows functional molecules to reach easily the NP surface [179,180].”

    From Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications (2020) · cites this paper as [179]

    The Experimental Section (SERS Methods; page unavailable) describes the study’s laser-ablated Ag and Au nanoparticles as “naked,” supporting the clean-surface aspect of the claim. It does not state that this makes the particles particularly desirable or that functional molecules can more easily reach their surfaces. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • In a collaboration testing LAL-produced NPs in 15 laboratories around Europe, a linear relationship between signals and analyte concentration was obtained with appropriate reproducibility and trueness, particularly with Au NPs.

    “The results were very satisfactory and show that, in particular with the Au NPs, a linear relationship between signals and analyte concentration was obtained with appropriate reproducibility and trueness [179].”

    From Nanoparticles Engineering by Pulsed Laser Ablation in Liquids: Concepts and Applications (2020) · cites this paper as [179]

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Authors
Fornasaro, S., Alsamad, F., Baia, M., Batista de Carvalho, L. A. E., Beleites, C., Byrne, H. J., Chiadò, A., Chis, M., Chisanga, M., Daniel, A., Dybas, J., Eppe, G., Falgayrac, G., Faulds, K., Gebavi, H., Giorgis, F., Goodacre, R., Graham, D., La Manna, P., Laing, S., Litti, L., Lyng, F. M., Malek, K., Malherbe, C., Marques, M. P. M., Meneghetti, M., Mitri, E., Mohaček-Grošev, V., Morasso, C., Muhamadali, H., Musto, P., Novara, C., Pannico, M., Penel, G., Piot, O., Rindzevicius, T., Rusu, E. A., Schmidt, M. S., Sergo, V., Sockalingum, G. D., Untereiner, V., Vanna, R., Wiercigroch, E., & Bonifacio, A.
Affiliations
  • University of Trieste (IT)
  • BioSpectroscopie Translationnelle (FR)
  • Babeș-Bolyai University (RO)
  • Molecular-Physical Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal
  • Chemometrix (Germany) (DE)
  • Technological University Dublin (IE)
  • Politecnico di Torino (IT)
  • Faculty of Physics, Babes-Bolyai University, M. Kogalniceanu 1, 400084 Cluj-Napoca, Romania
  • University of Manchester (GB)
  • Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, ul. Gronostajowa 2, 30-384 Krakow, Poland
  • University of Liège (BE)
  • Université de Lille (FR)
  • University of Strathclyde (GB)
  • Ruđer Bošković Institute (HR)
  • University of Liverpool (GB)
  • Institute on Polymers, Composites and Biomaterials, National Research Council of Italy, via Campi Flegrei, 34, Pozzuoli, Naples 80078, Italy
  • University of Padua (IT)
  • Mass Spectrometry Laboratory (MSLab), MolSys RU, University of Liège, Liège, Belgium
  • University of Coimbra (PT)
  • Centre of Excellence for Advanced Materials and Sensing Devices, Division of Materials Physics, Rudjer Boskovic Institute, Bijenicka c. 54, 10000 Zagreb, Croatia
  • Istituti Clinici Scientifici Maugeri (IT)
  • National Research Council (IT)
  • Technical University of Denmark (DK)
  • Silmeco ApS, Kenny Drews Vej 101, 2450 Copenhagen, Denmark
  • Jagiellonian University (PL)
Corresponding author
Bonifacio, Alois
ORCID
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
2020-02-11
Volume
92
Issue
5
Pages
4053-4064
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/acs.analchem.9b05658
PMC
PMC7997108
OpenAlex
W3006658492
Cited by
124
References
2
Citations by year
2026: 22 · 2025: 25 · 2024: 27 · 2023: 13 · 2022: 16 · 2021: 16 · 2020: 5
FWCI
8.61
Type
journal article
Language
en
Open access
Licence
Locations
  • Analytical Chemistry (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Strathprints: The University of Strathclyde institutional repository (University of Strathclyde) (acceptedVersion)
  • Homo Politicus (Academy of Humanities and Economics in Lodz) (submittedVersion, cc-by-nc-nd)
  • ArTS Archivio della ricerca di Trieste (University of Trieste https://www.units.it/) (submittedVersion, cc-by)
  • PORTO@Iris (submittedVersion, other-oa)
  • Padua Research Archive (University of Padova) (submittedVersion, cc-by)
  • HAL (submittedVersion, other-oa)
  • Institutional Repository of the Ruđer Bošković Institute (Ruđer Bošković Institute) (submittedVersion, other-oa)
  • ORBi (University of Liège) (submittedVersion)
  • Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion, cc-by)
Funders
  • Danmarks Grundforskningsfond (DNRF122)
  • Villum Fonden (9301)
  • European Cooperation in Science and Technology (BM1401)
  • Regione Piemonte (10808-178675264, 12866-2074-64512)
  • Minist??rio da Educa????o e Ci??ncia (UIDB/00070/2020)
  • Institute for Maternal and Child Health IRCCS Burlo Garofolo
Topics
  • Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
Keywords
surface-enhanced Raman spectroscopy, interlaboratory comparison, method validation, reproducibility, trueness, method comparison

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