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Cited by 63Animal Nutrition and PhysiologyAntimicrobial Peptides and ActivitiesVeterinary medicine and infectious diseases

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Different Lipopolysaccharide Branched-Chain Amino Acids Modulate Porcine Intestinal Endogenous β-Defensin Expression through the Sirt1/ERK/90RSK Pathway

Ren, Man, Zhang, Shihai, Liu, Xutong, Li, Shenghe, Mao, Xiangbing, Zeng, Xiangfang, Qiao, Shiyan·2016·Journal of Agricultural and Food Chemistry
DOI 10.1021/acs.jafc.6b00968
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Abstract

Nutritional induction of endogenous antimicrobial peptide expression is considered a promising approach to inhibit the outgrowth and infection of pathogenic microbes in mammals. The present study investigated possible regulation of porcine epithelial β-defensins in response to branched-chain amino acids (BCAA) in vivo and in vitro. BCAA treatment increased relative mRNA expression of jejunal and ileal β-defensins in weaned piglets. In IPEC-J2 cells, isoleucine, leucine, and valine could stimulate β-defensin expression, possibly associated with stimulation of ERK1/2 phosphorylation. Inhibition of Sirt1 and ERK completely blocked the activation of ERK and 90RSK protein by isoleucine, simultaneously decreasing defensin expression. BCAA stimulate expression of porcine intestinal epithelial β-defensins with isoleucine the most, potent possibly through activation of the Sirt1/ERK/90RSK signaling pathway. The β-defensins regulation of lipopolysaccharide was related with an ERK-independent pathway. BCAA modulation of endogenous defensin might be a promising approach to enhance disease resistance and intestinal health in young animals and children.

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Metadata

Authors
Ren, M., Zhang, S., Liu, X., Li, S., Mao, X., Zeng, X., & Qiao, S.
Affiliations
  • Anhui Science and Technology University (CN)
  • State Key Laboratory of Animal Nutrition (CN)
  • Sichuan Agricultural University (CN)
Corresponding author
  • Zeng, Xiangfang
  • Qiao, Shiyan
ORCID
Venue
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society (ACS)
Published
2016-04-25
Volume
64
Issue
17
Pages
3371-3379
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
DOI
10.1021/acs.jafc.6b00968
OpenAlex
W2347174969
Cited by
63
References
1
Citations by year
2026: 2 · 2025: 4 · 2024: 10 · 2023: 9 · 2022: 11 · 2021: 6 · 2020: 5 · 2019: 6 · 2018: 7 · 2017: 2 · 2016: 1
FWCI
7.57
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Science and Technology of the People's Republic of China (2012CB124704, 2013CB127305)
  • National Natural Science Foundation of China (31501968)
  • Natural Science Foundation of Anhui Province (1608085QC72)
  • Foundation of Anhui Province 115 Industrial Innovative Team
  • Foundation of Anhui Science and Technology University (ZRC2014453)
  • Anhui Province (KJ2015A296)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
  • Health Sciences › Veterinary › Small Animals › Veterinary medicine and infectious diseases
Keywords
branched-chain amino acids, lipopolysaccharide, beta-defensins, SIRT1, ERK signaling, isoleucine, intestinal epithelial cells, weaned piglets, antimicrobial peptides, disease resistance
MeSH terms
Amino Acids, Branched-Chain, Animals, Intestinal Mucosa, Intestines, Lipopolysaccharides, Male, Swine, MAP Kinase Signaling System, beta-Defensins, Sirtuin 1

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