Effect of Processing on the in Vitro and in Vivo Protein Quality of Yellow and Green Split Peas (Pisum sativum)
Abstract
In order to determine the effect of extrusion, baking, and cooking on the protein quality of yellow and green split peas, a rodent bioassay was conducted and compared to an in vitro method of protein quality determination. The Protein Digestibility-Corrected Amino Acid Score (PDCAAS) of green split peas (71.4%) was higher than that of yellow split peas (67.8%), on average. Similarly, the average Digestible Indispensable Amino Acid Score (DIAAS) of green split peas (69%) was higher than that of yellow split peas (67%). Cooked green pea flour had lower PDCAAS and DIAAS values (69.19% and 67%) than either extruded (73.61%, 70%) or baked (75.22%, 70%). Conversely, cooked yellow split peas had the highest PDCCAS value (69.19%), while extruded yellow split peas had the highest DIAAS value (67%). Interestingly, a strong correlation was found between in vivo and in vitro analysis of protein quality ( R 2 = 0.9745). This work highlights the differences between processing methods on pea protein quality and suggests that in vitro measurements of protein digestibility could be used as a surrogate for in vivo analysis.
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Animal proteins generally score highest on AAS due to their balanced amino acid profile, with casein ranging from 1.03 to 1.32.
“Animal proteins generally score highest due to their balanced amino acid profile, with casein ranging from 1.03 to 1.32 [27,28].”
From Navigating the potential of algal peptides: health effects, market applications, and scientific challenges (2026) · cites this paper as [27]
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- Authors
- Nosworthy, M. G., Franczyk, A. J., Medina, G., Neufeld, J., Appah, P., Utioh, A., Frohlich, P., & House, J. D.
- Affiliations
- University of Manitoba (CA)
- Food Development Centre, Portage la Prarie, MB R1N 3J9, Canada
- Canadian International Grains Institute (CA)
- Corresponding author
- House, James D.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-08-22
- Volume
- 65
- Issue
- 35
- Pages
- 7790-7796
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2745235649
- Cited by
- 99
- References
- 23
- Citations by year
- 2026: 11 · 2025: 11 · 2024: 19 · 2023: 17 · 2022: 9 · 2021: 16 · 2020: 6 · 2019: 7 · 2018: 3
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Global Institute for Food Security
- Agriculture and Agri-Food Canada - Growing Forward 2 Pulse Science Cluster (H32)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- Pisum sativum, protein digestibility, extrusion, baking, cooking, pea flour, PDCAAS, DIAAS, rodent bioassays
- MeSH terms
- Color, Cooking, Dietary Proteins, Digestion, Humans, Models, Biological, Nutritive Value, Plant Proteins, Seeds, Pisum sativum
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