The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Porcine Milk Exosome MiRNAs Attenuate LPS-Induced Apoptosis through Inhibiting TLR4/NF-κB and p53 Pathways in Intestinal Epithelial Cells
Abstract
Lipopolysaccharide (LPS) is a bacterial endotoxin that induces intestine inflammation. Milk exosomes improve the intestine and immune system development of newborns. This study aims to establish the protective mechanisms of porcine milk exosomes on the attenuation of LPS-induced intestinal inflammation and apoptosis. In vivo, exosomes prevented LPS-induced intestine damage and inhibited ( p < 0.05) LPS-induced inflammation. In vitro, exosomes inhibited ( p < 0.05) LPS-induced intestinal epithelial cells apoptosis (23% ± 0.4% to 12% ± 0.2%). Porcine milk exosomes also decreased ( p < 0.05) the LPS-induced TLR4/NF-κB signaling pathway activation. Furthermore, exosome miR-4334 and miR-219 reduced ( p < 0.05) LPS-induced inflammation through the NF-κB pathway and miR-338 inhibited ( p < 0.05) the LPS-induced apoptosis via the p53 pathway. Cotransfection with these three miRNAs more effectively prevented ( p < 0.05) LPS-induced cell apoptosis than these miRNAs individual transfection. The apoptosis percentage in the group cotransfected with the three miRNAs (14% ± 0.4%) was lower ( p < 0.05) than that in the NC miRNA group (28% ± 0.5%), and also lower than that in each individual miRNA group. In conclusion, porcine milk exosomes protect the intestine epithelial cells against LPS-induced injury by inhibiting cell inflammation and protecting against apoptosis through the action of exosome miRNAs. The presented results suggest that the physiological amounts of miRNAs-enriched exosomes addition to infant formula could be used as a novel preventative measure for necrotizing enterocolitis.
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Combinatorial microRNA target predictions
Krek, A., Grün, D., Poy, M. N., Wolf, R., Rosenberg, L., Epstein, E. J., MacMenamin, P., da Piedade, I., Gunsalus, K. C., Stoffel, M., et al. (2005) · Nature Genetics · doi:10.1038/ng1536 · Cited by 4,603
p53 Activates the CD95 (APO-1/Fas) Gene in Response to DNA Damage by Anticancer Drugs
Müller, M., Wilder, S., Bannasch, D., Israeli, D., Lehlbach, K., Li-Weber, M., Friedman, S. L., Galle, P. R., Stremmel, W., Oren, M., et al. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.11.2033 · Cited by 878
Dicer1 and miR-219 Are Required for Normal Oligodendrocyte Differentiation and Myelination
Dugas, J. C., Cuellar, T. L., Scholze, A., Ason, B., Ibrahim, A., Emery, B., Zamanian, J. L., Foo, L. C., McManus, M. T., & Barres, B. A. (2010) · Neuron · doi:10.1016/j.neuron.2010.01.027 · Cited by 577
miR-219 Cooperates with miR-338 in Myelination and Promotes Myelin Repair in the CNS
Wang, H., Moyano, A. L., Ma, Z., Deng, Y., Lin, Y., Zhao, C., Zhang, L., Jiang, M., He, X., Ma, Z., et al. (2017) · Developmental Cell · doi:10.1016/j.devcel.2017.03.001 · Cited by 174
Cited by
Tong, L., Hao, H., Zhang, Z., Lv, Y., Liang, X., Liu, Q., Liu, T., Gong, P., Zhang, L., Cao, F., et al. (2021) · Theranostics · doi:10.7150/thno.62046 · Cited by 325
Fan, X., Zhang, Y., Liu, W., Shao, M., Gong, Y., Wang, T., Xue, S., & Nian, R. (2024) · Biomaterials Science · doi:10.1039/d4bm00558a · Cited by 50
Self-citationShi, X.-J., Jin, Y., Xu, W.-M., Shen, Q., Li, J., & Chen, K. (2021) · Chinese Medical Journal · doi:10.1097/cm9.0000000000001369 · Cited by 11
Metadata
- Authors
- Xie, M.-Y., Hou, L.-J., Sun, J.-J., Zeng, B., Xi, Q.-Y., Luo, J.-Y., Chen, T., & Zhang, Y.-L.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-08-20
- Volume
- 67
- Issue
- 34
- Pages
- 9477-9491
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2969857495
- Cited by
- 183
- References
- 4
- FWCI
- 6.30
- Type
- journal article
- Language
- en
- Open access
- closed
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- Xie, M.-Y., Hou, L.-J., Sun, J.-J., Zeng, B., Xi, Q.-Y., Luo, J.-Y., Chen, T., & Zhang, Y.-L. (2019). Porcine Milk Exosome MiRNAs Attenuate LPS-Induced Apoptosis through Inhibiting TLR4/NF-κB and p53 Pathways in Intestinal Epithelial Cells. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/acs.jafc.9b02925
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