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Four phosphates at one blow: access to pentaphosphorylated magic spot nucleotides and their analysis by capillary electrophoresis.
Open accessAbstract
The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.
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The spc operon is under control of the rplN promoter and binding of RNA polymerase is inhibited when cells enter stationary phase by the transcription factor DksA and the alarmone ppGpp.
“The spc operon is under control of the rplN promotor and binding of RNA-polymerase is inhibited when cells enter stationary phase by the transcription factor DksA and the alarmone ppGpp, a hyper-phosphorylated guanosine derivative (Lemke et al., 2011; Haas et al., 2020).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Haas et al., 2020
The (p)ppGpp levels raise from approximately 40 μM during exponential phase up to approximately 1 mM at the transition into stationary phase or upon amino acid starvation.
“The (p)ppGpp levels raise from approx. 40 μM during exponential phase up to approx. 1 mM at the transition into stationary phase or upon amino acid starvation (Varik et al., 2017; Haas et al., 2020; Steinchen et al., 2020).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Haas et al., 2020
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Oswald, J., Njenga, R., Natriashvili, A., Sarmah, P., & Koch, H.-G. (2021) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2021.664241 · Cited by 88
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Metadata
- Authors
- Haas, T. M., Qiu, D., Häner, M., Angebauer, L., Ripp, A., Singh, J., Koch, H.-G., Jessen-Trefzer, C., & Jessen, H. J.
- Affiliations
- University of Freiburg (DE)
- University of Freiburg , , ,
- Corresponding author
- Jessen, Henning J.
- ORCID
- Venue
- The Journal of Organic Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-06-05
- Volume
- 85
- Issue
- 22
- Pages
- 14496-14506
- ISSN
- 0022-3263 (ISSN-L)
- 1520-6904
- PMC
- PMC7684580
- OpenAlex
- W3033326246
- Cited by
- 25
- References
- 48
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 1
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- The Journal of Organic Chemistry (publishedVersion, other-oa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (CIBBS ? EXC-2189, GRK2202, SFB1381)
- Studienstiftung des Deutschen Volkes
- Ministerium f?r Wissenschaft, Forschung und Kunst Baden-W?rttemberg
- H2020 European Research Council (864246)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Physical Sciences › Chemistry › Organic Chemistry › Organophosphorus compounds synthesis
- Physical Sciences › Chemistry › Organic Chemistry › Carbohydrate Chemistry and Synthesis
- Keywords
- nucleotide identification, bacterial extracts, nucleotide modification
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