Cited by 48Metalloenzymes and iron-sulfur proteinsEnzyme Structure and FunctionBioinformatics and Genomic Networks

Exploiting Bacterial Operons To Illuminate Human Iron–Sulfur Proteins

Andreini, Claudia, Banci, Lucia, Rosato, Antonio·2016·Journal of Proteome Research

Abstract

Organisms from all kingdoms of life use iron-sulfur proteins (FeS-Ps) in a multitude of functional processes. We applied a bioinformatics approach to investigate the human portfolio of FeS-Ps. Sixty-one percent of human FeS-Ps bind Fe4S4 clusters, whereas 39% bind Fe2S2 clusters. However, this relative ratio varies significantly depending on the specific cellular compartment. We compared the portfolio of human FeS-Ps to 12 other eukaryotes and to about 700 prokaryotes. The comparative analysis of the organization of the prokaryotic homologues of human FeS-Ps within operons allowed us to reconstruct the human functional networks involving the conserved FeS-Ps common to prokaryotes and eukaryotes. These functional networks have been maintained during evolution and thus presumably represent fundamental cellular processes. The respiratory chain and the ISC machinery for FeS-P biogenesis are the two conserved processes that involve the majority of human FeS-Ps. Purine metabolism is another process including several FeS-Ps, in which BOLA proteins possibly have a regulatory role. The analysis of the co-occurrence of human FeS-Ps with other proteins highlighted numerous links between the iron-sulfur cluster machinery and the response mechanisms to cell damage, from repair to apoptosis. This relationship probably relates to the production of reactive oxygen species within the biogenesis and degradation of FeS-Ps.

Integrity signals

Registry

Crossref · 10.1021/acs.jproteome.6b00045

Cites

86 of 86 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

1 retracted

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Andreini, C., Banci, L., & Rosato, A.
Affiliations
University of Florence (IT)
Corresponding author
Banci, Lucia
Venue
Journal of Proteome Research
Published
2016-03-02
Volume
15
Issue
4
Pages
1308-1322
ISSN
  • 1535-3893 (ISSN-L)
  • 1535-3907
OpenAlex
W2275585418
Cited by
48
References
86
Citations by year
2026: 1 · 2025: 5 · 2024: 4 · 2023: 6 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 9 · 2017: 7 · 2016: 4
FWCI
2.17
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Proteome Research (publishedVersion)
  • PubMed (publishedVersion)
  • Florence Research (University of Florence) (submittedVersion)
Funders
  • Ente Cassa di Risparmio di Firenze (2013/7201)
  • Ministero dell'Istruzione, dell'Università e della Ricerca (CTN01_00177_962865)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
Keywords
iron-sulfur proteins, bacterial operons, Fe4S4 cluster, [2Fe-2S] cluster, iron-sulfur cluster assembly, electron transport chain, purine metabolism, reactive oxygen species, apoptosis, comparative genomics
MeSH terms
Archaea, Bacteria, Bacterial Proteins, Cell Nucleus, Cytoplasm, Electron Transport, Biological Evolution, Humans, Iron-Sulfur Proteins, Mitochondria, Operon, Ribosomes, Saccharomyces cerevisiae, Species Specificity, User-Computer Interface, Gene Expression, Apoptosis, Arabidopsis, Reactive Oxygen Species, Protein Structure, Secondary, Computational Biology, Structural Homology, Protein

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review