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The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

Fluorescence Self-Quenching from Reporter Dyes Informs on the Structural Properties of Amyloid Clusters Formed in Vitro and in Cells

Chen, WeiYue, Young, Laurence J., Lu, Meng, Zaccone, Alessio, Ströhl, Florian, Yu, Na, Kaminski Schierle, Gabriele S., Kaminski, Clemens F.·2017·Nano Letters
DOI 10.1021/acs.nanolett.6b03686PMC PMC5338000
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Abstract

The characterization of the aggregation kinetics of protein amyloids and the structural properties of the ensuing aggregates are vital in the study of the pathogenesis of many neurodegenerative diseases and the discovery of therapeutic targets. In this article, we show that the fluorescence lifetime of synthetic dyes covalently attached to amyloid proteins informs on the structural properties of amyloid clusters formed both in vitro and in cells. We demonstrate that the mechanism behind such a “lifetime sensor” of protein aggregation is based on fluorescence self-quenching and that it offers a good dynamic range to report on various stages of aggregation without significantly perturbing the process under investigation. We show that the sensor informs on the structural density of amyloid clusters in a high-throughput and quantitative manner and in these aspects the sensor outperforms super-resolution imaging techniques. We demonstrate the power and speed of the method, offering capabilities, for example, in therapeutic screenings that monitor biological self-assembly. We investigate the mechanism and advantages of the lifetime sensor in studies of the K18 protein fragment of the Alzheimer’s disease related protein tau and its amyloid aggregates formed in vitro. Finally, we demonstrate the sensor in the study of aggregates of polyglutamine protein, a model used in studies related to Huntington’s disease, by performing correlative fluorescence lifetime imaging microscopy and structured-illumination microscopy experiments in cells.

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Cited by

Metadata

Authors
Chen, W., Young, L. J., Lu, M., Zaccone, A., Ströhl, F., Yu, N., Kaminski Schierle, G. S., & Kaminski, C. F.
Venue
Nano Letters
Publisher
American Chemical Society (ACS)
Published
2016-11-30
Volume
17
Issue
1
Pages
143-149
ISSN
  • 1530-6984
  • 1530-6992
DOI
10.1021/acs.nanolett.6b03686
PMC
PMC5338000
Cited by
85
References
5
Language
en
Licence
pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html (vor, 93 d)
Funders
  • Wellcome Trust (NA)
  • Medical Research Council (NA)
  • Engineering and Physical Sciences Research Council (NA)
  • Biotechnology and Biological Sciences Research Council (NA)
  • Alzheimer’s Research UK (NA)
  • Cambridge Commonwealth, European and International Trust (NA)
  • China Scholarship Council (NA)

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