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Therapeutic Efficacy-Potentiated and Diseased Organ-Targeting Nanovesicles Derived from Mesenchymal Stem Cells for Spinal Cord Injury Treatment
Abstract
Human mesenchymal stem cell (hMSC)-derived exosomes have been spotlighted as a promising therapeutic agent for cell-free regenerative medicine. However, poor organ-targeting ability and insufficient therapeutic efficacy of systemically injected hMSC-exosomes were identified as critical limitations for their further applications. Therefore, in this study we fabricated iron oxide nanoparticle (IONP)-incorporated exosome-mimetic nanovesicles (NV-IONP) from IONP-treated hMSCs and evaluated their therapeutic efficacy in a clinically relevant model for spinal cord injury. Compared to exosome-mimetic nanovesicles (NV) prepared from untreated hMSCs, NV-IONP not only contained IONPs which act as a magnet-guided navigation tool but also carried greater amounts of therapeutic growth factors that can be delivered to the target cells. The increased amounts of therapeutic growth factors inside NV-IONP were attributed to IONPs that are slowly ionized to iron ions which activate the JNK and c-Jun signaling cascades in hMSCs. In vivo systemic injection of NV-IONP with magnetic guidance significantly increased the amount of NV-IONP accumulating in the injured spinal cord. Accumulated NV-IONP enhanced blood vessel formation, attenuated inflammation and apoptosis in the injured spinal cord, and consequently improved spinal cord function. Taken together, these findings highlight the development of therapeutic efficacy-potentiated extracellular nanovesicles and demonstrate their feasibility for repairing injured spinal cord.
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“In a clinically relevant model of SCI, the systemic delivery of NV-IONP, directed by magnetic targeting, significantly increased their accumulation at the lesion site.”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [295]
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Metadata
- Authors
- Kim, H. Y., Kumar, H., Jo, M.-J., Kim, J., Yoon, J.-K., Lee, J.-R., Kang, M., Choo, Y. W., Song, S. Y., Kwon, S. P., Hyeon, T., Han, I.-B., & Kim, B.-S.
- Affiliations
- Seoul National University (KR)
- CHA University Bundang Medical Center (KR)
- Department of Neurosurgery, CHA University, CHA Bundang Medical Center, Seongnam-si, 13488, Republic of Korea
- Corresponding author
- Kim, Byung-Soo
- ORCID
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-07-11
- Volume
- 18
- Issue
- 8
- Pages
- 4965-4975
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W2825453881
- Cited by
- 190
- References
- 44
- Citations by year
- 2026: 9 · 2025: 16 · 2024: 32 · 2023: 27 · 2022: 34 · 2021: 37 · 2020: 23 · 2019: 9 · 2018: 3
- FWCI
- 6.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nano Letters (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Funders
- Korea Health Industry Development Institute (HI18C0183)
- National Research Foundation of Korea (2017R1A2B3005842, 2017R1C1B1011397)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Health Sciences › Medicine › Clinical Neurology › Spinal Cord Injury Research
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- mesenchymal stem cell-derived exosomes, iron oxide nanoparticles, magnetic targeting, spinal cord injury, JNK/c-Jun signaling, iron release, growth factor delivery, angiogenesis, neuroinflammation, apoptosis
- MeSH terms
- Animals, Drug Carriers, Humans, Spinal Cord Injuries, Signal Transduction, PC12 Cells, Apoptosis, Neovascularization, Physiologic, Intercellular Signaling Peptides and Proteins, Biomimetic Materials, Mice, Rats, Exosomes, Magnetite Nanoparticles, Mesenchymal Stem Cells, Drug Liberation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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