Analysis of Homotypic Interactions of Lactococcus lactis Pili Using Single-Cell Force Spectroscopy
Open accessAbstract
Cell surface proteins of Gram-positive bacteria play crucial roles in their adhesion to abiotic and biotic surfaces. Pili are long and flexible proteinaceous filaments known to enhance bacterial initial adhesion. They promote surface colonization and are thus considered as essential factors in biofilm cohesion. Our hypothesis is that pili mediate interactions between cells and may thereby directly affect biofilm formation. In this study, we use single-cell force spectroscopy (SCFS) to quantify the force of the homotypic pili interactions between individual bacterial cells, using different Lactococcus lactis strains producing pili or not as model bacteria. Moreover the force–distance curves were analyzed to determine the physical and nanomechanical properties of L. lactis pili. The results for pili-devoided strains showed a weak adhesion between cells (adhesion forces and work in the range of 100 pN and 7 × 10 –18 J, respectively). On the contrary, the piliated strains showed high adhesion levels with adhesion forces and adhesion work over 200 pN and 50 × 10 –18 J, respectively. The force–extension curves showed multiple adhesion events, typical of the unfolding of macromolecules. These unfolding force peaks were fitted using the physical worm-like chain model to get fundamental knowledge on the pili nanomechanical properties. In addition, SCFS applied to a L. lactis isolate expressing both pili and mucus-binding protein at its surface and two derivative mutants revealed the capacity of pili to interact with other surface proteins including mucus-binding proteins. This study demonstrates that pili are involved in L. lactis homotypic interactions and thus can influence biofilm structuring.
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Piliated L. lactis strains showed high adhesion force and work, whereas strains devoid of pili showed weak interactions.
“Both laboratory and environmental L. lactis showed a high adhesion force and work, in the case of piliated strains whereas strains devoid of pili showed weak interactions.”
From Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms (2021) · cites this paper as [25]
In a previous study, cell-cell adhesion abilities of L. lactis were driven by the presence of pili on their surface.
“we showed that cell–cell adhesion abilities were driven by the presence of pili on their surface.”
From Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms (2021) · cites this paper as [25]
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Drame, I., Lafforgue, C., Formosa-Dague, C., Chapot-Chartier, M.-P., Piard, J.-C., Castelain, M., & Dague, E. (2021) · Scientific Reports · doi:10.1038/s41598-021-84030-1 · Cited by 28 · see citing works
Self-citation
- Authors
- Dramé, I., Formosa-Dague, C., Lafforgue, C., Chapot-Chartier, M.-P., Piard, J.-C., Castelain, M., & Dague, E.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- TBI, Université de Toulouse, INSA, INRAE, CNRS, 31000 Toulouse, France
- Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350 Jouy-en-Josas, France
- AgroParisTech (FR)
- LAAS-CNRS, Université de Toulouse, CNRS, 31000 Toulouse, France
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-04-21
- Volume
- 12
- Issue
- 19
- Pages
- 21411-21423
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W3017201430
- Cited by
- 16
- References
- 45
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2023: 3 · 2021: 4 · 2020: 1
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- green
- hal.inrae.fr
- Licence
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HAL (submittedVersion, cc-by)
- Funders
- French ministry for research (Ibrahima Dram?? PhD Thesis)
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Lactococcus lactis, bacterial pili, bacterial adhesion, biofilm formation, single-cell force spectroscopy, worm-like chain model, force-distance curves, force-extension curves
- MeSH terms
- Bacterial Adhesion, Bacterial Proteins, Carrier Proteins, Cell Communication, Fimbriae, Bacterial, Lactococcus lactis, Microscopy, Atomic Force, Single-Cell Analysis
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