Single-cell force spectroscopy of the medically important Staphylococcus epidermidis–Candida albicans interaction
Open accessAbstract
Despite the clinical importance of bacterial-fungal interactions, their molecular details are poorly understood. A hallmark of such medically important interspecies associations is the interaction between the two nosocomial pathogens Staphylococcus aureus and Candida albicans, which can lead to mixed biofilm-associated infections with enhanced antibiotic resistance. Here, we use single-cell force spectroscopy (SCFS) to quantify the forces engaged in bacterial-fungal co-adhesion, focusing on the poorly investigated S. epidermidis-C. albicans interaction. Force curves recorded between single bacterial and fungal germ tubes showed large adhesion forces (~5 nN) with extended rupture lengths (up to 500 nm). By contrast, bacteria poorly adhered to yeast cells, emphasizing the important role of the yeast-to-hyphae transition in mediating adhesion to bacterial cells. Analysis of mutant strains altered in cell wall composition allowed us to distinguish the main fungal components involved in adhesion, i.e. Als proteins and O-mannosylations. We suggest that the measured co-adhesion forces are involved in the formation of mixed biofilms, thus possibly as well in promoting polymicrobial infections. In the future, we anticipate that this SCFS platform will be used in nanomedicine to decipher the molecular mechanisms of a wide variety of pathogen-pathogen interactions and may help in designing novel anti-adhesion agents.
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- Authors
- Beaussart, A., Herman, P., El-Kirat-Chatel, S., Lipke, P. N., Kucharíková, S., Van Dijck, P., & Dufrêne, Y. F.
- Affiliations
- UCLouvain (BE)
- City University of New York (US)
- VIB-KU Leuven Center for Microbiology (BE)
- Corresponding author
- Dufrêne, Yves F.
- Venue
- Nanoscale
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2013
- Volume
- 5
- Issue
- 22
- Pages
- 10894
- ISSN
- 2040-3364 (ISSN-L)
- 2040-3372
- PMC
- PMC3825105
- OpenAlex
- W1983972612
- Cited by
- 95
- References
- 37
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 7 · 2021: 11 · 2020: 8 · 2019: 13 · 2018: 3 · 2017: 9 · 2016: 5 · 2015: 14 · 2014: 8 · 2013: 2
- FWCI
- 4.97
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nanoscale (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DIAL (Catholic University of Leuven) (submittedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM098616)
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Staphylococcus epidermidis, Candida albicans, yeast-to-hypha transition, germ tube, O-mannosylation, cell wall composition, polymicrobial biofilms, co-infection, nosocomial pathogens, anti-adhesion agents
- MeSH terms
- Anti-Bacterial Agents, Bacterial Adhesion, Candida albicans, Glycoconjugates, Glycoproteins, Staphylococcus epidermidis, Surface Properties, Biofilms, Microscopy, Atomic Force, Nanomedicine
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