Bacterial photoinactivation using PLGA electrospun scaffolds
Abstract
The use of ultraviolet (UV) and blue irradiation to sterilize surfaces is well established, but commercial applications would be enhanced if the light source is replaced with ambient light. In this paper, it is shown that nanofibers can be explored as an alternative methodology to UV and blue irradiation for bacterial inactivation. It is demonstrated that this is indeed possible using spun nanofibers of poly[lactic- co -(glycolic acid)] (PLGA). This work shows that PLGA spun scaffolds can promote photoinactivation of Staphylococcus aureus and Escherichia coli bacteria with ambient light or with laser irradiation at 630 nm. With the optimized scaffold composition of PLGA85:15 nanofibers, the minimum intensity required to kill the bacteria is much lower than in antimicrobial blue light applications. The enhanced effect introduced by PLGA scaffolds is due to their nanofiber structures since PLGA spun nanofibers were able to inactivate both S. aureus and E. coli bacteria, but cast films had no effect. These findings pave the way for an entirely different method to sterilize surfaces, which is less costly and environmentally friendly than current procedures. In addition, the scaffolds could also be used in cancer treatment with fewer side effects since photosensitizers are not required.
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Electrospun PLGA fibers stimulate cell-matrix interaction to form a cell niche, direct cellular behavior, and promote MSCs adhesion and proliferation.
“Electrospun PLGA fibers are capable of stimulating cell-matrix interaction to form a cell niche, directing cellular behavior, and promoting the MSCs adhesion and proliferation [34,35].”
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- Authors
- Pereira, A. O., Lopes, I. M. I., Silva, T. R., Corrêa, T. Q., Paschoalin, R. T., Inada, N. M., Iermak, I., van Riel Neto, F., Araujo-Chaves, J. C., Marletta, A., Tozoni, J. R., Mattoso, L. H. C., Bagnato, V. S., Nantes-Cardoso, I. L., Oliveira, O. N., & Campana, P. T.
- Affiliations
- Universidade de São Paulo (BR)
- Sao Carlos Institute of Physics, University of São Paulo (USP), Trabalhador São-Carlense Av., 400, Sao Carlos 13560-970, Brazil
- Institute of Physics, Federal University of Uberlândia (UFU), João Naves de Ávila Av., 2121, Uberlândia 38408-100, Brazil
- Universidade Federal do ABC (BR)
- Universidade Federal de Uberlândia (BR)
- Embrapa (BR)
- Center of Natural Sciences and HumanitiesFederal University of ABC (UFABC), dos Estados Av., 5001, Santo André 09210-580, Brazil
- Corresponding author
- Campana, Patricia T.
- ORCID
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2021-06-29
- Volume
- 13
- Issue
- 27
- Pages
- 31406-31417
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W3176982704
- Cited by
- 10
- References
- 58
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 2 · 2022: 4
- FWCI
- 1.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Funda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (2010/06728-8, 2013/03609-6, 2013/14262-7, 2017/18725-2, 2018/22214-6)
- Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior (001)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Keywords
- PLGA nanofibers, electrospun scaffolds, surface sterilization, Staphylococcus aureus, Escherichia coli, ambient light, laser irradiation, 630 nm red light, nanofiber structure
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Electricity, Escherichia coli, Staphylococcus aureus, Ultraviolet Rays, Microbial Viability
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