Improving Osteointegration and Osteogenesis of Three-Dimensional Porous Ti6Al4V Scaffolds by Polydopamine-Assisted Biomimetic Hydroxyapatite Coating
Abstract
Titanium alloys with various porous structures can be fabricated by advanced additive manufacturing techniques, which are attractive for use as scaffolds for bone defect repair. However, modification of the scaffold surfaces, particularly inner surfaces, is critical to improve the osteointegration of these scaffolds. In this study, a biomimetic approach was employed to construct polydopamine-assisted hydroxyapatite coating (HA/pDA) onto porous Ti6Al4V scaffolds fabricated by the electron beam melting method. The surface modification was characterized with the field emission scanning electron microscopy, energy dispersive spectroscopy, water contact angle measurement, and confocal laser scanning microscopy. Attachment and proliferation of MC3T3-E1 cells on the scaffold surface were significantly enhanced by the HA/pDA coating compared to the unmodified surfaces. Additionally, MC3T3-E1 cells grown on the HA/pDA-coated Ti6Al4V scaffolds displayed significantly higher expression of runt-related transcription factor-2, alkaline phosphatase, osteocalcin, osteopontin, and collagen type-1 compared with bare Ti6Al4V scaffolds after culture for 14 days. Moreover, microcomputed tomography analysis and Van-Gieson staining of histological sections showed that HA/pDA coating on surfaces of porous Ti6Al4V scaffolds enhanced osteointegration and significantly promoted bone regeneration after implantation in rabbit femoral condylar defects for 4 and 12 weeks. Therefore, this study provides an…
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HA/PDA coating on porous Ti6Al4V scaffolds boosts osteointegration and endorses bone regeneration in rabbit femoral condylar defects after 12 weeks by approximately one fold.
“Moreover, in vitro studies revealed that HA/PDA coating on surfaces of porous Ti6Al4V scaffolds boosted osteointegration and endorsed bone regeneration in rabbit femoral condylar defects after implantation for 12 weeks by approximately one fold (Figure 5) [55].”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [55]
MC3T3-E1 cells grown on HA/PDA-coated Ti6Al4V scaffolds show upregulation of osteogenic genes Runx2 (~1-fold), ALP (~1-fold), OCN (~1-fold), OPN (~1.5-fold), and collagen type-1 (~1.5-fold) compared with bare Ti6Al4V scaffolds after 14 days of culture.
“MC3T3-E1 grown on the HA/PDA-coated Ti6Al4V scaffolds showed upregulation of osteogenic genes, such as Runx2 (~1-fold), ALP (~1-fold), OCN (~1-fold), OPN (~1.5-fold), and collagen type-1 (~1.5-fold) compared with bare Ti6Al4V scaffolds after 14 days of culture.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [55]
HA/PDA coating on porous Ti6Al4V scaffolds enhances attachment and proliferation of MC3T3-E1 cells by approximately 25% compared to non-modified surfaces.
“They stated that the attachment and proliferation of mouse preosteoblast cells (MC3T3-E1) were approximately 25% enhanced by the HA/PDA coating on the scaffold surface as compared to the non-modified surfaces.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [55]
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Mussel‐Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization
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Normal Bone Anatomy and Physiology
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“Reprinted with permission from reference [55].”
BackgroundMethodInfluentialPolydopamine Applications in Biomedicine and Environmental Science
Omidian, H., & Wilson, R. L. (2024) · Materials · doi:10.3390/ma17163916 · Cited by 47 · see citing works
- Authors
- Li, Y., Yang, W., Li, X., Zhang, X., Wang, C., Meng, X., Pei, Y., Fan, X., Lan, P., Wang, C., Li, X., & Guo, Z.
- Affiliations
- Xijing Hospital (CN)
- Chinese Academy of Sciences (CN)
- Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, People’s Republic of China
- Corresponding author
- Guo, Zheng
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-03-03
- Volume
- 7
- Issue
- 10
- Pages
- 5715-5724
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2319009721
- Cited by
- 210
- References
- 2
- Citations by year
- 2026: 11 · 2025: 19 · 2024: 16 · 2023: 22 · 2022: 29 · 2021: 15 · 2020: 29 · 2019: 18 · 2018: 19 · 2017: 13 · 2016: 13 · 2015: 3
- FWCI
- 7.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Science and Technology of the People's Republic of China (2015AA033702)
- National Natural Science Foundation of China (51271199, 81171773)
- Shaanxi Province (2012KTCG01-14)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
- Physical Sciences › Materials Science › Materials Chemistry › Titanium Alloys Microstructure and Properties
- Keywords
- Ti-6Al-4V, porous scaffolds, polydopamine coating, hydroxyapatite coating, biomimetic surface modification, osseointegration, bone formation, electron beam melting, bone regeneration, MC3T3-E1 cells, bone defect repair, orthopedic implants
- MeSH terms
- Adsorption, Alloys, Animals, Indoles, Materials Testing, Osteogenesis, Polymers, Prosthesis Design, Titanium, Porosity, Osseointegration, Equipment Failure Analysis, Coated Materials, Biocompatible, Biomimetic Materials, BALB 3T3 Cells, Cell Proliferation, Mice, Tissue Scaffolds
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