The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Cholesterol Biosynthesis and Uptake in Developing Neurons
Open accessAbstract
Brain cholesterol biosynthesis, a separate and distinct process from whole-body cholesterol homeostasis, starts during embryonic development. To gain a better understanding of the neuronal and glial contributions to the brain cholesterol pool, we studied this process in control, Dhcr7 –/–, and Dhcr24 –/– cell cultures. Our LC-MS/MS method allowed us to measure several different sterol intermediates and cholesterol during neuronal differentiation. We found that developing cortical neurons rely on endogenous cholesterol synthesis and utilize ApoE-complexed cholesterol and sterol precursors from their surroundings. Both developing neurons and astrocytes release cholesterol into their local environment. Our studies also uncovered that developing neurons produced significantly higher amounts of cholesterol per cell than the astrocytes. Finally, we established that both neurons and astroglia preferentially use the Bloch sterol biosynthesis pathway, where desmosterol is the immediate precursor to cholesterol. Overall, our studies suggest that endogenous sterol synthesis in developing neurons is a critical and complexly regulated homeostatic process during brain development.
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“Astrocytes produce excess cholesterol and secrete most of the produced cholesterol for use by neurons7.”
From An iPSC-derived astrocyte model of fragile X syndrome exhibits dysregulated cholesterol homeostasis (2023) · cites this paper as [7]
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Cholesterol metabolism and homeostasis in the brain
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Bloch, K. (1965) · Science · doi:10.1126/science.150.3692.19 · Cited by 562
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Waterham, H. R., Koster, J., Romeijn, G. J., Hennekam, R. C. M., Vreken, P., Andersson, H. C., FitzPatrick, D. R., Kelley, R. I., & Wanders, R. J. A. (2001) · The American Journal of Human Genetics · doi:10.1086/323473 · Cited by 355
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Cholesterol and Alzheimer’s Disease; From Risk Genes to Pathological Effects
Feringa, F. M., & van der Kant, R. (2021) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2021.690372 · Cited by 226
Lipid metabolism and storage in neuroglia: role in brain development and neurodegenerative diseases
Yang, D., Wang, X., Zhang, L., Fang, Y., Zheng, Q., Liu, X., Yu, W., Chen, S., Ying, J., & Hua, F. (2022) · Cell & Bioscience · doi:10.1186/s13578-022-00828-0 · Cited by 179
Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory
Li, X., Zhang, J., Li, D., He, C., He, K., Xue, T., Wan, L., Zhang, C., & Liu, Q. (2021) · Neuron · doi:10.1016/j.neuron.2021.01.005 · Cited by 165
Goldstein, L. B., Toth, P. P., Dearborn, J. L., Giugliano, R. P., Hirsh, B. J., Peña, J. M., Selim, M. H., & Woo, D. (2023) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atv.0000000000000164 · Cited by 99
Impaired cholesterol metabolism, neurons, and neuropsychiatric disorders
Cheon, S. Y. (2023) · Experimental Neurobiology · doi:10.5607/en23010 · Cited by 67
Metadata
- Authors
- Genaro-Mattos, T. C., Anderson, A., Allen, L. B., Korade, Z., & Mirnics, K.
- Affiliations
- Munroe-Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, Nebraska 68105, United States
- University of Nebraska Medical Center (US)
- Department of Pediatrics, College of Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198, United States
- Corresponding author
- Mirnics, Károly
- Venue
- ACS Chemical Neuroscience
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-06-07
- Volume
- 10
- Issue
- 8
- Pages
- 3671-3681
- ISSN
- 1948-7193 (ISSN-L)
- PMC
- PMC7184320
- OpenAlex
- W2950398114
- Cited by
- 117
- References
- 49
- Citations by year
- 2026: 17 · 2025: 21 · 2024: 19 · 2023: 18 · 2022: 22 · 2021: 12 · 2020: 8
- FWCI
- 10.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- ACS Chemical Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Mental Health (R01 MH110636, R01 MH067234)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Keywords
- cholesterol absorption, developing neurons, cortical neurons, astrocytes, DHCR7, DHCR24, sterol intermediates, desmosterol, neuronal differentiation, brain development, cholesterol homeostasis
- MeSH terms
- Animals, Astrocytes, Biological Transport, Brain, Cholesterol, Chromatography, Liquid, Neurons, Sterols, Mice, Tandem Mass Spectrometry
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