Spray Drying and Crystallization of Cottonseed Meal Raffinose as a New Prebiotic
Abstract
Isolated raffinose could have beneficial effects as a prebiotic in the prevention of cardio vascular diseases and is considered to be protective against colonic diseases such as ulcerative colitis and possibly also colon cancer. Cottonseed meal (CSM) is a byproduct of the cotton oil industry and has high raffinose content. New Mexico State University is utilizing a new variety of cottonseed that is gossypol free for high-quality feed and human food products. The long-term goal of this project is to obtain a method to extract raffinose from the glandless CSM. CSM with two different fat concentrations (6.52 and 13.33%) was studied. Raffinose was precipitated with a 50% ethanol solution and crystallized with a 50% acetone solution or spray-dried. Low fat content CSM showed 6.6% of raffinose, while high fat content CSM had only 2.4%. After extraction and concentration, crystallized raffinose had a higher ( p <0.05) concentration than spray-dried raffinose. Further studies are aimed to increase the raffinose extraction rate and purity in the final product. Purified raffinose can be used to develop new novel foods with a commercial and consumer application worldwide.
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Trypsin inhibitors, raffinose, and phytic acid are associated with reduced cancer risk.
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From Valorization of Microalgae as Multifunctional Protein Sources in Plant-Based Systems: Structural, Functional, and Processing Challenges (2026) · cites this paper as [39]
Cites
Prebiotics: Present and future in food science and technology
Wang, Y. (2008) · Food Research International · doi:10.1016/j.foodres.2008.09.001 · Cited by 316 · see citing works
Content of raffinose oligosaccharides and sucrose in various plant seeds
Kuo, T. M., VanMiddlesworth, J. F., & Wolf, W. J. (1988) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00079a008 · Cited by 283 · see citing works
Múzquiz, M., Varela, A., Burbano, C., Cuadrado, C., Guillamón, E., & Pedrosa, M. M. (2012) · Phytochemistry Reviews · doi:10.1007/s11101-012-9233-9 · Cited by 215 · see citing works
Spray Dried Products—Characterization of Particle Morphology
Walton, D. E., & Mumford, C. (1999) · Process Safety and Environmental Protection · doi:10.1205/026387699525846 · Cited by 210 · see citing works
Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol
Tada, N., Sato, M., YAMANOI, J., et al. (1990) · Reproduction · doi:10.1530/jrf.0.0890511 · Cited by 208 · see citing works
Characterization of polysaccharides extracted from spent coffee grounds by alkali pretreatment
Ballesteros, L. F., Cerqueira, M. Â., Teixeira, J. A., & Mussatto, S. I. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.03.047 · Cited by 199 · see citing works
León‐Martínez, F. M., Méndez‐Lagunas, L. L., & Rodríguez‐Ramírez, J. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.03.061 · Cited by 173 · see citing works
Soybean - Biochemistry, Chemistry and Physiology
Ng, T. (2011) · InTech eBooks · doi:10.5772/1952 · Cited by 140 · see citing works
Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots
Azmi, A. F. M. N., Mustafa, S., Hashim, D. M., & Manap, Y. A. (2012) · Molecules · doi:10.3390/molecules17021635 · Cited by 124 · see citing works
Structural characterization of polysaccharides from Hyriopsis cumingii
Qiao, D., Liu, J., Ke, C., Sun, Y., Ye, H., & Zeng, X. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.06.048 · Cited by 121 · see citing works
Chen, H., Liu, L., Zhu, J., Xu, B., & Li, R. (2009) · Food Chemistry · doi:10.1016/j.foodchem.2009.09.056 · Cited by 111 · see citing works
Jia, X., Zhang, C., Qiu, J., Wang, L., Bao, J., Wang, K., Zhang, Y., Chen, M., Wan, J., Su, H., et al. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.05.059 · Cited by 103 · see citing works
Carbohydrates, Dietary Fiber, and Resistant Starch in White Vegetables: Links to Health Outcomes
Slavin, J. L. (2013) · Advances in Nutrition · doi:10.3945/an.112.003491 · Cited by 84 · see citing works
Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats
Watanabe, H., Sonoyama, K., Watanabe, J., Yamaguchi, N., Kikuchi, H., Nagura, T., Aritsuka, T., Fukumoto, K., & Kasai, T. (2004) · British Journal Of Nutrition · doi:10.1079/bjn20041179 · Cited by 52 · see citing works
Lupins as a potential source of raffinose family oligosaccharides
Múzquiz, M., Burbano, C., Pedrosa, M. M., et al. (1999) · Industrial Crops and Products · doi:10.1016/s0926-6690(98)00030-2 · Cited by 50 · see citing works
Bello, B., Mustafa, S., Tan, J. S., et al. (2018) · 3 Biotech · doi:10.1007/s13205-018-1362-4 · Cited by 48 · see citing works
Abbasiliasi, S., Tan, J. S., Ibrahim, T. A. T., et al. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.11.019 · Cited by 40 · see citing works
Bourne, E. J., Walter, M., & Pridham, J. B. (1965) · Biochemical Journal · doi:10.1042/bj0970802 · Cited by 31 · see citing works
Abbasiliasi, S., Tan, J. S., Bello, B., et al. (2019) · Biotechnology & Biotechnological Equipment · doi:10.1080/13102818.2019.1649603 · Cited by 20 · see citing works
Zhu, X., Liu, J., & Yang, G. (2021) · Animal Science Journal · doi:10.1111/asj.13668 · Cited by 20 · see citing works
Effect of Spray Drying of Agave Fructans, Nopal Mucilage and Aloe Vera Juice
Farías-Cervantes, V. S., Chávez‐Rodríguez, A., Delgado, E., et al. (2016) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.13027 · Cited by 13 · see citing works
Churchill, T. A., Green, C. J., Davidson, B. R., et al. (1995) · Transplant International · doi:10.1111/j.1432-2277.1995.tb01538.x · Cited by 13 · see citing works
Sigvardson, K. W., Eliason, M. S., & Herbranson, D. E. (1996) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/0731-7085(96)01842-0 · Cited by 9 · see citing works
Zhao, Y., Niu, S., Huang, L., et al. (2013) · Tetrahedron · doi:10.1016/j.tet.2013.03.094 · Cited by 7 · see citing works
Isolation of raffinose from cottonseed meal
Иванова, И., Турахожаев, М. Т., & Shakirov, T. T. (1984) · Chemistry of Natural Compounds · doi:10.1007/bf00580014 · Cited by 5 · see citing works
Gomyô, T., & NAKAMURA, M. (1966) · Agricultural and Biological Chemistry · doi:10.1080/00021369.1966.10858618 · Cited by 3 · see citing works
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Boza, A., Lemos, E., Bürck, M., Gautério, G. V., & Braga, A. R. C. (2026) · Macromol · doi:10.3390/macromol6030054
- Authors
- Rodríguez, A. C., Nancy, F. C., Efrén, D.-L., Hiram, M.-R., Isaac, A.-G., & Cervantes, V. S. F.
- Affiliations
- Universidad Politécnica de la Zona Metropolitana de Guadalajara (MX)
- New Mexico State University (US)
- Department of Extension Family and Consumer Sciences
- Tecnologico Nacional de México Campus Durango, Felipe Pescador 1830 Oriente, Nueva Vizcaya, Durango, Dgo CP 34080, Mexico
- Tecnológico Nacional de México Campus Tlajomulco, Km 10 Circuito Metropolitano Sur, Tlajomulco de Zúñiga, Jal CP 45640, Mexico
- Venue
- ACS Food Science & Technology
- Publisher
- American Chemical Society (ACS)
- Published
- 2025-08-25
- Volume
- 5
- Issue
- 9
- Pages
- 3317-3322
- ISSN
- 2692-1944 (ISSN-L)
- OpenAlex
- W4413581751
- Cited by
- 2
- References
- 26
- Citations by year
- 2026: 2
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Food Science & Technology (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Microencapsulation and Drying Processes
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Keywords
- raffinose, cottonseed meal, prebiotics, spray drying, crystallization, fat content, novel foods, colonic health
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