Enzyme-Powered Hollow Nanorobots for Active Microsampling Enabled by Thermoresponsive Polymer Gating
Abstract
Achieving molecular sample capture at micro/nanoscales while integrating functions of controllable loading and real-time monitoring of cargo molecules is of great significance in the development of intelligent micro/nanorobots. Herein, we prepare a temperature-responsive microsampling nanorobot by encapsulating metal (Au) nanodots inside hollow mesoporous silica nanoparticles and grafting a temperature-responsive polymer, poly( N -isopropylacrylamide), on their external surface. The molecular gate of nanochannels accessing the internal hollow reservoir can be switched between “open” and “closed” states by regulating the temperature, allowing on-demand loading and releasing of small molecules. The internally embedded surface-enhanced Raman scattering hotspots of gold nanodots can serve as sensing probes for real-time detection of the molecular cargo load inside the hollow nanorobots. Furthermore, we demonstrate temperature-dependent self-propulsion behavior of the nanorobots driven by enzymatic reactions. The active motion behavior can favorably regulate the loading efficiency of molecular cargos. In addition, by further introducing the magnetic component Ni, the nanorobots can accomplish effective transportation of cargo molecules by magnetic guidance under real-time Raman monitoring. The current strategy is expected to provide a manipulable nanorobot platform for precise biomedical sampling, which holds promising potential for disease diagnosis or controlled drug delivery in precision medicine.
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The poly(N-isopropylacrylamide) grafted hollow MSN allows the nanochannels access the internal hollow reservoir to be switched between 'open' and 'closed' states by regulating the temperature, allowing on-demand loading and releasing of small molecules.
“The poly(N-isopropylacrylamide) grafted hollow MSN allows the nanochannels access the internal hollow reservoir to be switched between “open” and “closed” states by regulating the temperature, allowing on-demand loading and releasing of small molecules.74”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [74]
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Smart nanoparticles for cancer therapy
Sun, L., Liu, H., Ye, Y., Lei, Y., Islam, R., Tan, S., Tong, R., Miao, Y.-B., & Cai, L. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01642-x · Cited by 1,073 · see citing works
- Authors
- Liu, X., Chen, W., Zhao, D., Liu, X., Wang, Y., Chen, Y., & Ma, X.
- Affiliations
- Harbin Institute of Technology (CN)
- Corresponding author
- Chen, Wenjun
- Ma, Xing
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2022-07-11
- Volume
- 16
- Issue
- 7
- Pages
- 10354-10363
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W4285022426
- Cited by
- 50
- References
- 38
- Citations by year
- 2026: 5 · 2025: 14 · 2024: 19 · 2023: 11 · 2022: 1
- FWCI
- 4.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Nano (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Shenzhen Bay Laboratory (SZBL2019062801005)
- Central University Basic Research Fund of China (HIT.OCEF.2021032)
- Science, Technology and Innovation Commission of Shenzhen Municipality (JCYJ20200109113408066, KQTD20170809110344233)
- National Natural Science Foundation of China (52072095, 92163109)
- Topics
- Physical Sciences › Physics and Astronomy › Condensed Matter Physics › Micro and Nano Robotics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Keywords
- poly(N-isopropylacrylamide), mesoporous silica nanoparticles, gold nanodots, surface-enhanced Raman scattering, magnetic guidance, controlled drug delivery
- MeSH terms
- Gold, Polymers, Silicon Dioxide, Drug Delivery Systems, Nanoparticles
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