The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Surface Functionalization of Nanoparticles with Polyethylene Glycol: Effects on Protein Adsorption and Cellular Uptake
Open accessAbstract
Here we have investigated the effect of enshrouding polymer-coated nanoparticles (NPs) with polyethylene glycol (PEG) on the adsorption of proteins and uptake by cultured cells. PEG was covalently linked to the polymer surface to the maximal grafting density achievable under our experimental conditions. Changes in the effective hydrodynamic radius of the NPs upon adsorption of human serum albumin (HSA) and fibrinogen (FIB) were measured in situ using fluorescence correlation spectroscopy. For NPs without a PEG shell, a thickness increase of around 3 nm, corresponding to HSA monolayer adsorption, was measured at high HSA concentration. Only 50% of this value was found for NPs with PEGylated surfaces. While the size increase clearly reveals formation of a protein corona also for PEGylated NPs, fluorescence lifetime measurements and quenching experiments suggest that the adsorbed HSA molecules are buried within the PEG shell. For FIB adsorption onto PEGylated NPs, even less change in NP diameter was observed. In vitro uptake of the NPs by 3T3 fibroblasts was reduced to around 10% upon PEGylation with PEG chains of 10 kDa. Thus, even though the PEG coatings did not completely prevent protein adsorption, the PEGylated NPs still displayed a pronounced reduction of cellular uptake with respect to bare NPs, which is to be expected if the adsorbed proteins are not exposed on the NP surface.
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Cites
Monopoli, M. P., Walczyk, D., Campbell, A., Elia, G., Lynch, I., Baldelli Bombelli, F., & Dawson, K. A. (2011) · Journal of the American Chemical Society · doi:10.1021/ja107583h · Cited by 1,835
A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
Ostuni, E., Chapman, R. G., Holmlin, R. E., Takayama, S., & Whitesides, G. M. (2001) · Langmuir · doi:10.1021/la010384m · Cited by 1,799
Duan, X., & Li, Y. (2013) · Small · doi:10.1002/smll.201201390 · Cited by 926
Treuel, L., Brandholt, S., Maffre, P., Wiegele, S., Shang, L., & Nienhaus, G. U. (2014) · ACS Nano · doi:10.1021/nn405019v · Cited by 419
Self-citationPEG-Graft Density Controls Polymeric Nanoparticle Micelle Stability
Logie, J., Owen, S. C., McLaughlin, C. K., & Shoichet, M. S. (2014) · Chemistry of Materials · doi:10.1021/cm500448x · Cited by 110
Cited by
Chen, Z., Zhao, P., Luo, Z., Zheng, M., Tian, H., Gong, P., Gao, G., Pan, H., Liu, L., Ma, A., et al. (2016) · ACS Nano · doi:10.1021/acsnano.6b04695 · Cited by 895
A Decade of the Protein Corona
Ke, P. C., Lin, S., Parak, W. J., Davis, T. P., & Caruso, F. (2017) · ACS Nano · doi:10.1021/acsnano.7b08008 · Cited by 672
Self-citationComplement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo
Chen, F., Wang, G., Griffin, J. I., Brenneman, B., Banda, N. K., Holers, V. M., Backos, D. S., Wu, L., Moghimi, S. M., & Simberg, D. (2017) · Nature Nanotechnology · doi:10.1038/nnano.2016.269 · Cited by 599
Xu, Q., Ensign, L. M., Boylan, N. J., Schön, A., Gong, X., Yang, J.-C., Lamb, N. W., Cai, S., Yu, T., Freire, E., et al. (2015) · ACS Nano · doi:10.1021/acsnano.5b03876 · Cited by 568
In vivo degeneration and the fate of inorganic nanoparticles
Feliu, N., Docter, D., Heine, M., del Pino, P., Ashraf, S., Kolosnjaj-Tabi, J., Macchiarini, P., Nielsen, P., Alloyeau, D., Gazeau, F., et al. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00699f · Cited by 423
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Metadata
- Authors
- Pelaz, B., del Pino, P., Maffre, P., Hartmann, R., Gallego, M., Rivera-Fernández, S., de la Fuente, J. M., Nienhaus, G. U., & Parak, W. J.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-06-25
- Volume
- 9
- Issue
- 7
- Pages
- 6996-7008
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2402319960
- Cited by
- 890
- References
- 5
- FWCI
- 29.82
- Type
- journal article
- Language
- en
- Open access
- green
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- Pelaz, B., del Pino, P., Maffre, P., Hartmann, R., Gallego, M., Rivera-Fernández, S., de la Fuente, J. M., Nienhaus, G. U., & Parak, W. J. (2015). Surface Functionalization of Nanoparticles with Polyethylene Glycol: Effects on Protein Adsorption and Cellular Uptake. ACS Nano. https://doi.org/10.1021/acsnano.5b01326
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