Cited by 1,799Polymer Surface Interaction StudiesSurface Chemistry and CatalysisSurface Modification and Superhydrophobicity

A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein

Ostuni, Emanuele, Chapman, Robert G., Holmlin, R. Erik, Takayama, Shuichi, Whitesides, George M.·2001·Langmuir

Abstract

This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific adsorption of proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N -acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the adsorption of proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the adsorption of proteins. In the group of surfaces examined, those that resisted the adsorption of proteins had the following properties: they were hydrophilic; they contained groups that were hydrogen-bond acceptors but not hydrogen-bond donors; and they were overall electrically neutral.

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Crossref · 10.1021/la010384m

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Authors
Ostuni, E., Chapman, R. G., Holmlin, R. E., Takayama, S., & Whitesides, G. M.
Affiliations
Harvard University (US)
Venue
Langmuir
Published
2001-07-31
Volume
17
Issue
18
Pages
5605-5620
ISSN
  • 0743-7463 (ISSN-L)
  • 1520-5827
OpenAlex
W2045260522
Cited by
1,799
References
54
Citations by year
2026: 28 · 2025: 60 · 2024: 66 · 2023: 65 · 2022: 69 · 2021: 72 · 2020: 91 · 2019: 76 · 2018: 83 · 2017: 97 · 2016: 97 · 2015: 103 · 2014: 110 · 2013: 92 · 2012: 108
FWCI
20.12
Type
journal article
Language
en
Open access
closed
Locations
Langmuir (publishedVersion)
Topics
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Physical Sciences › Engineering › Biomedical Engineering › Surface Chemistry and Catalysis
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Surface Modification and Superhydrophobicity
Keywords
protein adsorption resistance, nonspecific protein adsorption, self-assembled monolayers, mixed self-assembled monolayers, surface plasmon resonance, oligo(ethylene glycol), surface hydrophilicity, hydrogen bond acceptors, structure-property relationships

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