A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
Abstract
This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific adsorption of proteins from solution. Mixed SAMs presenting different functional groups were prepared for screening using a synthetic protocol based on the reaction of organic amines with a SAM terminated by interchain carboxylic anhydride groups. Surfaces that presented derivatives of oligo(sarcosine), N -acetylpiperazine, and permethylated sorbitol groups were particularly effective in resisting the adsorption of proteins. Incorporation of these groups into single-component SAMs resulted in surfaces that are comparable to (but slightly less good than) single-component SAMs that present oligo(ethylene glycol) in their ability to resist the adsorption of proteins. In the group of surfaces examined, those that resisted the adsorption of proteins had the following properties: they were hydrophilic; they contained groups that were hydrogen-bond acceptors but not hydrogen-bond donors; and they were overall electrically neutral.
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Biomaterials Science: An Introduction to Materials in Medicine
Ratner, B. D., Hoffman, A. S., Schöen, F. J., & Lemons, J. E. (1996) · doi:10.1016/c2009-0-02433-7 · Cited by 5,125 · see citing works
Printing Proteins as Microarrays for High-Throughput Function Determination
MacBeath, G., & Schreiber, S. L. (2000) · Science · doi:10.1126/science.289.5485.1760 · Cited by 2,767 · see citing works
Self-Assembled Organic Monolayers: Model Systems for Studying Adsorption of Proteins at Aurfaces
Prime, K. L., & Whitesides, G. M. (1991) · Science · doi:10.1126/science.252.5009.1164 · Cited by 1,834 · see citing works
Self-citationFunctional Arteries Grown in Vitro
Niklason, L. E., Gao, J., Abbott, W. M., Hirschi, K. K., Houser, S. L., Marini, R. P., & Langer, R. (1999) · Science · doi:10.1126/science.284.5413.489 · Cited by 1,767 · see citing works
Prime, K. L., & Whitesides, G. M. (1993) · Journal of the American Chemical Society · doi:10.1021/ja00076a032 · Cited by 1,629 · see citing works
Self-citationEngineering cell shape and function
Singhvi, R., Kumar, A., Lopez, G. P., Stephanopoulos, G. N., Wang, D. I. C., Whitesides, G. M., & Ingber, D. E. (1994) · Science · doi:10.1126/science.8171320 · Cited by 1,453 · see citing works
Self-citationHarder, P., Grunze, M., Dahint, R., Whitesides, G. M., & Laibinis, P. E. (1998) · The Journal of Physical Chemistry B · doi:10.1021/jp972635z · Cited by 1,291 · see citing works
Self-citationZwitterionic SAMs that Resist Nonspecific Adsorption of Protein from Aqueous Buffer
Holmlin, R. E., Chen, X., Chapman, R., Takayama, S., & Whitesides, G. M. (2001) · Langmuir · doi:10.1021/la0015258 · Cited by 892 · see citing works
Self-citationA controlled-release microchip
Santini, J. T., Cima, M. J., & Langer, R. (1999) · Nature · doi:10.1038/16898 · Cited by 866 · see citing works
Whitesides, G. M., & Laibinis, P. E. (1990) · Langmuir · doi:10.1021/la00091a013 · Cited by 856 · see citing works
Self-citationEffect of Surface Wettability on the Adsorption of Proteins and Detergents
Sigal, G. B., Mrksich, M., & Whitesides, G. M. (1998) · Journal of the American Chemical Society · doi:10.1021/ja970819l · Cited by 818 · see citing works
Self-citationArginine-Mediated RNA Recognition: the Arginine Fork
Calnan, B. J., Tidor, B., Biancalana, S., Hudson, D., & Frankel, A. D. (1991) · Science · doi:10.1126/science.252.5009.1167 · Cited by 746 · see citing works
The Global Spread of Malaria in a Future, Warmer World
Rogers, D. J., & Randolph, S. (2000) · Science · doi:10.1126/science.289.5485.1763 · Cited by 701 · see citing works
Surveying for Surfaces that Resist the Adsorption of Proteins
Chapman, R., Ostuni, E., Takayama, S., Holmlin, R. E., Yan, L., & Whitesides, G. M. (2000) · Journal of the American Chemical Society · doi:10.1021/ja000774f · Cited by 600 · see citing works
Self-citationLahiri, J., Isaacs, L., Tien, J., & Whitesides, G. M. (1999) · Analytical Chemistry · doi:10.1021/ac980959t · Cited by 599 · see citing works
Self-citationBoschker, H. T. S., Nold, S. C., Wellsbury, P., Bos, D., de Graaf, W., Pel, R., Parkes, R. J., & Cappenberg, T. E. (1998) · Nature · doi:10.1038/33900 · Cited by 556 · see citing works
Sigal, G. B., Bamdad, C. C., Barberis, A., et al. (1996) · Analytical Chemistry · doi:10.1021/ac9504023 · Cited by 498 · see citing works
Höök, F., Rodahl, M., Kasemo, B., et al. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.21.12271 · Cited by 483 · see citing works
Dense Populations of a Giant Sulfur Bacterium in Namibian Shelf Sediments
Schulz, H. N., Brinkhoff, T., Ferdelman, T. G., Mariné, M. H., Teske, A., & Jørgensen, B. B. (1999) · Science · doi:10.1126/science.284.5413.493 · Cited by 481 · see citing works
Structural changes in proteins adsorbed on polymer surfaces
Soderquist, M. E., & Walton, A. G. (1980) · Journal of Colloid and Interface Science · doi:10.1016/0021-9797(80)90463-4 · Cited by 452 · see citing works
Fragments of the HIV-1 Tat Protein Specifically Biand TAR RNA
Weeks, K. M., Ampè, C., Schultz, S. C., Steitz, T. A., & Crothers, D. M. (1990) · Science · doi:10.1126/science.2205002 · Cited by 445 · see citing works
McPherson, T. B., Kidane, A., Szleifer, I. G., & Park, K. (1998) · Langmuir · doi:10.1021/la9706781 · Cited by 434 · see citing works
Protein Adsorption on Surfaces with Grafted Polymers
Szleifer, I. G. (1997) · Biophysical Journal · doi:10.1016/s0006-3495(97)78698-3 · Cited by 427 · see citing works
Malmsten, M. (1998) · CRC Press eBooks · doi:10.1201/9780824746391 · Cited by 410 · see citing works
Oxidation of polyethylene glycols by alcohol dehydrogenase
Herold, D. A., Keil, K., & Bruns, D. E. (1989) · Biochemical Pharmacology · doi:10.1016/0006-2952(89)90151-2 · Cited by 403 · see citing works
Akihiro, I., & Sanes, J. R. (1997) · Science · doi:10.1126/science.276.5317.1428 · Cited by 388 · see citing works
Polymeric Thin Films That Resist the Adsorption of Proteins and the Adhesion of Bacteria
Chapman, R., Ostuni, E., Liang, M. N., Meluleni, G. J., Kim, E., Yan, L., Pier, G. B., Warren, H. S., & Whitesides, G. M. (2001) · Langmuir · doi:10.1021/la001222d · Cited by 356 · see citing works
Self-citationMicrosystem Technology in Chemistry and Life Science
Manz, A., & Becker, H. (1998) · Topics in current chemistry · doi:10.1007/3-540-69544-3 · Cited by 347 · see citing works
Mrksich, M., Sigal, G. B., & Whitesides, G. M. (1995) · Langmuir · doi:10.1021/la00011a034 · Cited by 342 · see citing works
Self-citationFeldman, K., Hähner, G., Spencer, N. D., et al. (1999) · Journal of the American Chemical Society · doi:10.1021/ja991049b · Cited by 279 · see citing works
Thiel, A. J., Frutos, A. G., Jordan, C. E., et al. (1997) · Analytical Chemistry · doi:10.1021/ac9708001 · Cited by 270 · see citing works
Chapman, R., Ostuni, E., Yan, L., et al. (2000) · Langmuir · doi:10.1021/la991579l · Cited by 254 · see citing works
Self-citationKinetic and thermodynamic control of protein adsorption
Satulovsky, J. E., Carignano, M. A., & Szleifer, I. G. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.150236197 · Cited by 253 · see citing works
Pertsin, A. J., & Grunze, M. (2000) · Langmuir · doi:10.1021/la000340y · Cited by 220 · see citing works
Polymers and proteins: interactions at interfaces
Szleifer, I. G. (1997) · Current Opinion in Solid State and Materials Science · doi:10.1016/s1359-0286(97)80125-8 · Cited by 209 · see citing works
Evidence for bromine monoxide in the free troposphere during the Arctic polar sunrise
McElroy, C. T., McLinden, C. A., & McConnell, J. C. (1999) · Nature · doi:10.1038/16904 · Cited by 166 · see citing works
Zolk, M., Eisert, F., Pipper, J., et al. (2000) · Langmuir · doi:10.1021/la0003239 · Cited by 155 · see citing works
Theory of Hydration Forces between Surfaces
Besseling, N. A. M. (1997) · Langmuir · doi:10.1021/la960672w · Cited by 146 · see citing works
Deible, C. R., Petrosko, P., Johnson, P. C., Beckman, E. J., Russell, A. J., & Wagner, W. R. (1998) · Biomaterials · doi:10.1016/s0142-9612(98)00098-2 · Cited by 100 · see citing works
Protein adsorption on tethered polymer layers: effect of polymer chain architecture and composition
Szleifer, I. G. (1997) · Physica A Statistical Mechanics and its Applications · doi:10.1016/s0378-4371(97)00293-8 · Cited by 99 · see citing works
Healing of segmental bone defects in rats induced by a ?-TCP-MCPM cement combined with rhBMP-2
Ohura, K., Hamanishi, C., Tanaka, S., & Matsuda, N. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199902)44:2 · Cited by 77 · see citing works
Sastre‐Santos, Á., Díaz‐García, M. A., del Rey, B., et al. (1997) · The Journal of Physical Chemistry A · doi:10.1021/jp972361f · Cited by 76 · see citing works
A genome-wide functional assay of signal transduction in living mammalian cells
Whitney, M., Rockenstein, E. M., Cantin, G. T., et al. (1998) · Nature Biotechnology · doi:10.1038/4302 · Cited by 67 · see citing works
Aldehyde-Terminated Self-Assembled Monolayers on Gold: Immobilization of Amines onto Gold Surfaces
Horton, R. C., Herne, T. M., & Myles, D. C. (1997) · Journal of the American Chemical Society · doi:10.1021/ja971329w · Cited by 65 · see citing works
Autoxidation of Pyridoxalated Hemoglobin Polyoxyethylene Conjugate
Talarico, T. L., Swank, A., & Privalle, C. T. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9312 · Cited by 39 · see citing works
Li, P., & Brueggemeier, R. W. (1990) · Journal of Medicinal Chemistry · doi:10.1021/jm00163a017 · Cited by 37 · see citing works
Near-Infrared Spectral Studies for Investigating the Hydration of 18-Crown-6 in Aqueous Solutions
Patil, K. J., & Pawar, R. B. (1999) · The Journal of Physical Chemistry B · doi:10.1021/jp983548p · Cited by 37 · see citing works
Dissociation equilibrium and potentiometric titration of .beta.-lactoglobulin acidic solutions
Holtzer, A. M., & Nagasawa, M. (1971) · Journal of the American Chemical Society · doi:10.1021/ja00732a008 · Cited by 33 · see citing works
Macrophages and cutaneous inflammation
McCormick, T. S., Stevens, S. R., & Kang, K. (2000) · Nature Biotechnology · doi:10.1038/71879 · Cited by 26 · see citing works
Reactions of water-soluble metalloporphyrins with the serum protein, hemopexin
Gibbs, E. J., Skowronek, W. R., Morgan, W. T., Müller‐Eberhard, U., & Pasternack, R. F. (1980) · Journal of the American Chemical Society · doi:10.1021/ja00531a042 · Cited by 22 · see citing works
Cravey, M. J., & Kohn, H. (1980) · Journal of the American Chemical Society · doi:10.1021/ja00531a041 · Cited by 19 · see citing works
Gerhardt, W., & Martens, C. (1985) · Zeitschrift für Chemie · doi:10.1002/zfch.19850250412 · Cited by 19 · see citing works
Divergent Surface Functionalization Using Acid Fluoride-Functionalized Self-Assembled Monolayers
Niemz, A., Jeoung, E., Boal, A. K., et al. (1999) · Langmuir · doi:10.1021/la990972o · Cited by 14 · see citing works
Hj, K., Speiser, P., & Soliva, M. (1975) · PubMed · Cited by 5 · see citing works
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Slaughter, B. V., Khurshid, S. S., Fisher, O. Z., Khademhosseini, A., & Peppas, N. A. (2009) · Advanced Materials · doi:10.1002/adma.200802106 · Cited by 2,703 · see citing works
Zwitterionic hydrogels implanted in mice resist the foreign-body reaction
Zhang, L., Cao, Z., Bai, T., Carr, L., Ella-Menye, J.-R., Irvin, C., Ratner, B. D., & Jiang, S. (2013) · Nature Biotechnology · doi:10.1038/nbt.2580 · Cited by 1,035 · see citing works
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Xu, L.-C., & Siedlecki, C. A. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.03.032 · Cited by 715 · see citing works
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Kotov, N. A., Winter, J. O., Clements, I. P., Jan, E., Timko, B. P., Campidelli, S., Pathak, S., Mazzatenta, A., Lieber, C. M., Prato, M., et al. (2009) · Advanced Materials · doi:10.1002/adma.200801984 · Cited by 504 · see citing works
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Xu, L.-C., Bauer, J. W., & Siedlecki, C. A. (2014) · Colloids and Surfaces B: Biointerfaces · doi:10.1016/j.colsurfb.2014.09.040 · Cited by 391 · see citing works
A Switchable Biocompatible Polymer Surface with Self‐Sterilizing and Nonfouling Capabilities
Cheng, G., Xue, H., Zhang, Z., Chen, S., & Jiang, S. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200803570 · Cited by 371 · see citing works
Wo, Y., Brisbois, E. J., Bartlett, R. H., & Meyerhoff, M. E. (2016) · Biomaterials Science · doi:10.1039/c6bm00271d · Cited by 251 · see citing works
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- Authors
- Ostuni, E., Chapman, R. G., Holmlin, R. E., Takayama, S., & Whitesides, G. M.
- Affiliations
- Harvard University (US)
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-07-31
- Volume
- 17
- Issue
- 18
- Pages
- 5605-5620
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2045260522
- Cited by
- 1,799
- References
- 54
- Citations by year
- 2026: 28 · 2025: 60 · 2024: 66 · 2023: 65 · 2022: 69 · 2021: 72 · 2020: 91 · 2019: 76 · 2018: 83 · 2017: 97 · 2016: 97 · 2015: 103 · 2014: 110 · 2013: 92 · 2012: 108
- FWCI
- 20.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Langmuir (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Physical Sciences › Engineering › Biomedical Engineering › Surface Chemistry and Catalysis
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Surface Modification and Superhydrophobicity
- Keywords
- protein adsorption resistance, nonspecific protein adsorption, self-assembled monolayers, mixed self-assembled monolayers, surface plasmon resonance, oligo(ethylene glycol), surface hydrophilicity, hydrogen bond acceptors, structure-property relationships
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