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Sticky Matrix: Adhesion Mechanism of the Staphylococcal Polysaccharide Intercellular Adhesin
Abstract
The development of bacterial biofilms on surfaces leads to hospital-acquired infections that are difficult to fight. In Staphylococci, the cationic polysaccharide intercellular adhesin (PIA) forms an extracellular matrix that connects the cells together during biofilm formation, but the molecular forces involved are unknown. Here, we use advanced force nanoscopy techniques to unravel the mechanism of PIA-mediated adhesion in a clinically relevant methicillin-resistant Staphylococcus aureus (MRSA) strain. Nanoscale multiparametric imaging of the structure, adhesion, and elasticity of bacteria expressing PIA shows that the cells are surrounded by a soft and adhesive matrix of extracellular polymers. Cell surface softness and adhesion are dramatically reduced in mutant cells deficient for the synthesis of PIA or under unfavorable growth conditions. Single-cell force spectroscopy demonstrates that PIA promotes cell-cell adhesion via the multivalent electrostatic interaction with polyanionic teichoic acids on the S. aureus cell surface. This binding mechanism rationalizes, at the nanoscale, the well-known ability of PIA to strengthen intercellular adhesion in staphylococcal biofilms. Force nanoscopy offers promising prospects for understanding the fundamental forces in antibiotic-resistant biofilms and for designing anti-adhesion compounds targeting matrix polymers.
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In some cases, carbohydrates, particularly exopolysaccharides, can act as autoagglutinins.
“In some cases, also carbohydrates, particularly exopolysaccharides, can act as autoagglutinins.”
From Bacterial autoaggregation (2018) · cites this paper as [16]
The polysaccharide intercellular adhesin (PNAG) of staphylococci is an example of an exopolysaccharide agglutinin.
“An example of an exopolysaccharide agglutinin is the polysaccharide intercellular adhesin (poly-N-acetylglucosamine; PNAG) of staphylococci [16].”
From Bacterial autoaggregation (2018) · cites this paper as [16]
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“the polysaccharide intercellular adhesin (poly-N-acetylglucosamine; PNAG) of staphylococci [16].”
Background
Metadata
- Authors
- Formosa-Dague, C., Feuillie, C., Beaussart, A., Derclaye, S., Kucharíková, S., Lasa, I., Van Dijck, P., & Dufrêne, Y. F.
- Affiliations
- Institute of Life Sciences, Université catholique de Louvain, Croix du Sud, 4-5, bte L7.07.06., B-1348 Louvain-la-Neuve, Belgium
- UCLouvain (BE)
- VIB-KU Leuven Center for Microbiology (BE)
- Group of Microbial Communities and Disease, Navarrabiomed-FMS, UPNA, IdiSNA, 31008 Navarra, Spain
- Walloon Excellence in Lifesciences and Biotechnology (BE)
- Corresponding author
- Dufrêne, Yves F.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-02-23
- Volume
- 10
- Issue
- 3
- Pages
- 3443-3452
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2283231348
- Cited by
- 103
- References
- 41
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 7 · 2023: 10 · 2022: 15 · 2021: 6 · 2020: 12 · 2019: 11 · 2018: 12 · 2017: 8 · 2016: 6
- FWCI
- 6.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Nano (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- DIAL (Catholic University of Leuven) (submittedVersion)
- Lirias (submittedVersion)
- Funders
- Federaal Wetenschapsbeleid
- Fonds Wetenschappelijk Onderzoek
- Fonds De La Recherche Scientifique - FNRS (WELBIO-CR-2015A-05)
- Communaut? fran?aise de Belgique
- Spanish Ministry of Economy and Competitiveness (BIO2014-53530-R)
- WELBIO (WELBIO-CR-2015A-05)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Keywords
- polysaccharide intercellular adhesin, PIA, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, bacterial biofilms, cell-cell adhesion, extracellular matrix, teichoic acid, electrostatic interactions, force spectroscopy, nosocomial infections
- MeSH terms
- Bacterial Adhesion, Humans, Polysaccharides, Bacterial, Staphylococcal Infections, Staphylococcus aureus, Biofilms, Microscopy, Atomic Force
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