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Cited by 373

The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.

Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

Cheng, Ziyi, Choi, Namhyun, Wang, Rui, Lee, Sangyeop, Moon, Kyung Chul, Yoon, Soo-Young, Chen, Lingxin, Choo, Jaebum·2017·ACS Nano

Abstract

Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.

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Metadata

Authors
Cheng, Z., Choi, N., Wang, R., Lee, S., Moon, K. C., Yoon, S.-Y., Chen, L., & Choo, J.
Venue
ACS Nano
Published
2017-04-27
Volume
11
Issue
5
Pages
4926-4933
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
OpenAlex
W2608064079
Cited by
373
References
17
FWCI
14.19
Type
journal article
Language
en
Open access
closed

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