Innovating Generation of Nanocellulose from Industrial Hemp by Dual Asymmetric Centrifugation

Agate, Sachin; Tyagi, Preeti; Naithani, Ved; Lucia, Lucian; Pal, Lokendra

doi:10.1021/acssuschemeng.9b05992

Cited by 52Advanced Cellulose Research StudiesLignin and Wood ChemistryNanocomposite Films for Food Packaging

Innovating Generation of Nanocellulose from Industrial Hemp by Dual Asymmetric Centrifugation

Agate, Sachin, Tyagi, Preeti, Naithani, Ved, Lucia, Lucian, Pal, Lokendra·2020·ACS Sustainable Chemistry & Engineering

DOI 10.1021/acssuschemeng.9b05992

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Abstract

Among nanobiomaterials, cellulose nanofibrils (CNF) possessing intrinsically appealing fiber dimensions on the nanometer scale and biocompatibility feature arguably the greatest potential for a variety of practical applications such as packaging, hygiene, food, and healthcare products. Herein, a new class of nanofibers are introduced from a very green feedstock using an isolated mechanical processing technique, enabling on-site and on-demand production with a significantly reduced risk of environmental and toxicological concerns during the product life cycle. Serendipitously, we have shown that autohydrolyzed hemp hurds, the short fiber piths of the hemp plant, show a surprising and unparalleled tendency to generate nanocellulose. Hemp hurd fibers were pulverized using a high intensity dual asymmetric centrifugation (DAC) force to defibrillate unbleached hemp cellulosic fibers into lignin-containing cellulose micro and nanofibers (L-CMNF). The impact of biomass composition, chemical processing, and mechanical pretreatment as well as DAC pulverization time and solids content on the fibrillation, crystallinity, charge, and colloidal stability of L-CMNF were studied. The formation of L-CMNF caused reduced crystallinity from 50% → 29% while charge distribution increased from 4.5 → 13 (× 10 –5 μ eq Vol./L of p-DADMAC) with higher pulverization time at lower solids in the presence of lignin. Finally, SEM and TEM images substantiated the formation of L-CMNF. Thus, we offer a powerful new approach to CNF generation by pulverizing autohydrolyzed hemp hurds for very short times in an isolated DAC system.

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The short fiber length of hemp hurds provides a distinct advantage to produce nanocellulose biopolymers in terms of energy consumption.
“The short fiber length of hemp hurds provides a distinct advantage to produce nanocellulose biopolymers in terms of energy consumption (Agate et al. 2020).”
From A critical review of industrial fiber hemp anatomy, agronomic practices, and valorization into sustainable bioproducts (2025) · cites this paper as Agate et al. 2020

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10.15376/biores.7.1.0422-0436 · DOI
10.15376/biores.12.1.2143-2233 · DOI
Edyta M., 2015, Annals of Warsaw University of Life Sciences - SGGW Forestry and Wood Technology, 91, 134

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Authors: Agate, S., Tyagi, P., Naithani, V., Lucia, L., & Pal, L.
Affiliations: North Carolina State University (US)
Corresponding author: Pal, Lokendra
ORCID: Agate, Sachin
Tyagi, Preeti
Naithani, Ved
Lucia, Lucian
Pal, Lokendra
Venue: ACS Sustainable Chemistry & Engineering
Publisher: American Chemical Society (ACS)
Published: 2020-01-07
Volume: 8
Issue: 4
Pages: 1850-1858
ISSN: 2168-0485 (ISSN-L)
DOI: 10.1021/acssuschemeng.9b05992
OpenAlex: W2998963064
Semantic Scholar: ec17e28ed34a6ae393f6de7f5a17f98363af6931
Cited by: 51
References: 63
Citations by year: 2026: 5 · 2025: 6 · 2024: 6 · 2023: 11 · 2022: 7 · 2021: 12 · 2020: 3
FWCI: 2.15
Type: journal article
Language: en
Open access: closed
Licence: doi.org/10.15223/policy-029 (stm-asf)
doi.org/10.15223/policy-037 (stm-asf)
doi.org/10.15223/policy-045 (stm-asf)
Locations: ACS Sustainable Chemistry & Engineering (publishedVersion)
Topics: Physical Sciences › Materials Science › Biomaterials › Advanced Cellulose Research Studies
Physical Sciences › Engineering › Biomedical Engineering › Lignin and Wood Chemistry
Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
Keywords: nanocellulose, cellulose nanofibers, industrial hemp, hemp hurd, mechanical fibrillation, autohydrolysis, cellulose crystallinity, surface charge, colloidal stability, nanobiomaterials, lignocellulosic biomass

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Innovating Generation of Nanocellulose from Industrial Hemp by Dual Asymmetric Centrifugation

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