The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation
Open accessAbstract
-cleavage activity against collateral single-stranded DNA (ssDNA) and employed the activity to develop a rapid nucleic acid detection system, namely HOLMES (one-hour low-cost multipurpose highly efficient system). Here, with the employment of thermophilic CRISPR-Cas12b, we create HOLMESv2 for four different applications: (1) specifically discriminating single nucleotide polymorphism (SNP); (2) simply detecting virus RNA, human cell mRNA and circular RNA; (3) conveniently quantitating target nucleic acids with a one-step system combined with LAMP amplification in a constant temperature, thus avoiding cross-contamination; (4) accurately quantitating target DNA methylation degree with the combination of Cas12b detection and bisulfite treatment. These results highlight the potential of HOLMESv2 as a promising platform for both molecular diagnostics and epigenetics applications.
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Citations
Cites
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aar6245 · Cited by 4,507
Function and information content of DNA methylation
Schübeler, D. (2015) · Nature · doi:10.1038/nature14192 · Cited by 2,060
Cited by
SHERLOCK: nucleic acid detection with CRISPR nucleases
Kellner, M. J., Koob, J. G., Gootenberg, J. S., Abudayyeh, O. O., & Zhang, F. (2019) · Nature Protocols · doi:10.1038/s41596-019-0210-2 · Cited by 1,631
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
CRISPR/Cas Systems towards Next-Generation Biosensing
Li, Y., Li, S., Wang, J., & Liu, G. (2019) · Trends in Biotechnology · doi:10.1016/j.tibtech.2018.12.005 · Cited by 917
Self-citationUltrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis
Liu, Y., Zhan, L., Qin, Z., Sackrison, J., & Bischof, J. C. (2021) · ACS Nano · doi:10.1021/acsnano.0c10035 · Cited by 626
Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies
Aman, R., Mahas, A., & Mahfouz, M. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00507 · Cited by 409
Metadata
- Authors
- Li, L., Li, S., Wu, N., Wu, J., Wang, G., Zhao, G., & Wang, J.
- Venue
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-09-18
- Volume
- 8
- Issue
- 10
- Pages
- 2228-2237
- ISSN
- 2161-5063 (ISSN-L)
- OpenAlex
- W2974267223
- Cited by
- 793
- References
- 2
- FWCI
- 17.76
- Type
- journal article
- Language
- en
- Open access
- green
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