Galactose Targeted pH-Responsive Copolymer Conjugated with Near Infrared Fluorescence Probe for Imaging of Intelligent Drug Delivery
Abstract
Theranostic polymeric nanomaterials are of special important in cancer treatment. Here, novel galactose targeted pH-responsive amphiphilic multiblock copolymer conjugated with both drug and near-infrared fluorescence (NIR) probe has been designed and prepared by a four-steps process: (1) ring-opening polymerization (ROP) of N-carboxy anhydride (NCA) monomers using propargylamine as initiator; (2) reversible addition-fragmentation chain transfer (RAFT) polymerization of oligo(ethylene glycol) methacrylate (OEGMA) and gal monomer by an azido modified RAFT agent; (3) combing the obtained two polymeric segments by click reaction; (4) NIR copolymer prodrug was synthesized by chemical linkage of both cyanine dye and anticancer drug doxorubicin to the block copolymer via amide bond and hydrazone, respectively. The obtained NIRF copolymers were characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), and its was measured by means of micelles dynamic light scattering (DLS), field emission transmission electron microscopy (FETEM), and UV-vis and fluorescence spectrophotometry. The prodrug has strong fluorescence in the near-infrared region, and a pH sensitive drug release was confirmed at pH of 5.4 via an in vitro drug release experiment. Confocal laser scanning microscopy (CLSM) and flow cytometry experiments of the prodrug on both HepG2 and NIH3T3 cells reveal that the galactose targeted polymeric prodrug shows a fast and enhanced endocytosis due to the specific interaction for HepG2 cells, indicating the as-prepared polymer is a candidate for theranosis of liver cancer.
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A pH-responsive multiblock amphiphilic copolymer with galactose-targeted moiety was recently developed, which also incorporated NIR dye indocyanine green probe and therapeutic unit and demonstrated high uptake specifically to HepG2 cells.
“a pH-responsive multiblock amphiphilic copolymer with galactose-targeted moiety was recently developed, which also incorporated NIR dye indocyanine green probe and therapeutic unit and demonstrated high uptake specifically to HepG2 cells [186].”
From Recent Advances in Nanomicelles Delivery Systems. (2020) · cites this paper as [186]
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pH-sensitive biomaterials for drug delivery
Zhuo, S., Zhang, F., Yu, J., Zhang, X., Yang, G., & Liu, X. (2020) · Molecules · doi:10.3390/molecules25235649 · Cited by 247 · see citing works
Recent Advances in Nanomicelles Delivery Systems.
Tawfik, S. M., Azizov, S., Elmasry, M. R., Sharipov, M., & Lee, Y.-I. (2020) · Nanomaterials · doi:10.3390/nano11010070 · Cited by 156 · see citing works
“Likewise, a pH-responsive multiblock amphiphilic copolymer with galactose-targeted moiety was recently developed, which also incorporated NIR dye indocyanine green probe and therapeutic unit and demonstrated high uptake specifically to HepG2 cells [186].”
Background
- Authors
- Fu, L., Sun, C., & Yan, L.
- Affiliations
- University of Science and Technology of China (CN)
- Corresponding author
- Yan, Lifeng
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-01-16
- Volume
- 7
- Issue
- 3
- Pages
- 2104-2115
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2322075178
- Cited by
- 78
- References
- 64
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 6 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 12 · 2018: 15 · 2017: 7 · 2016: 15 · 2015: 2
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (51373162)
- Ministry of Science and Technology of the People's Republic of China (2011CB921403)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
- Keywords
- near-infrared fluorescent probe, RAFT polymerization, click chemistry, doxorubicin prodrug, cyanine dyes, hydrazone linkage, micelles, endocytosis, liver cancer, theranostics, HepG2 cells
- MeSH terms
- Animals, Carbon Dioxide, Doxorubicin, Drug Carriers, Fluorescence, Galactose, Humans, Polymers, Molecular Probes, Drug Delivery Systems, NIH 3T3 Cells, Mice, Hep G2 Cells, Molecular Imaging, Polymerization
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