The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Different Strategies for Formation of PEGylated EGF-Conjugated PEI/DNA Complexes for Targeted Gene Delivery
Abstract
With the aim of generating gene delivery systems for tumor targeting, we have synthesized a conjugate consisting of polyethylenimine (PEI) covalently modified with epidermal growth factor (EGF) peptides. Transfection efficiency of the conjugate was evaluated and compared to native PEI in three tumor cell lines: KB epidermoid carcinoma cells, CMT-93 rectum carcinoma cells, and Renca-EGFR renal carcinoma cells. Depending on the tumor cell line, incorporation of EGF resulted in an up to 300-fold increased transfection efficiency. This ligand-mediated enhancement and competition with free EGF strongly suggested uptake of the complexes through the EGF receptor-mediated endocytosis pathway. Shielded particles being crucial for systemic gene delivery, we studied the effect of covalent surface modification of EGF-PEI/DNA complexes with a poly(ethylene glycol) (PEG) derivative. An alternative way for the formation of PEGylated EGF-containing complexes was also evaluated where EGF was projected away from PEI/DNA core complexes through a PEG linker. Both strategies led to shielded particles still able to efficiently transfect tumor cells in a receptor-dependent fashion. These PEGylated EGF-containing complexes were 10- to 100-fold more efficient than PEGylated complexes without EGF.
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Citations
Cites
Ogris, M., Brunner, S., Schüller, S., Kircheis, R., & Wagner, E. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300900 · Cited by 1,178
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Cited by
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Park, J. H., Lee, S., Kim, J.-H., Park, K., Kim, K., & Kwon, I. C. (2008) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2007.09.003 · Cited by 506
Non-viral gene delivery systems
Davis, M. E. (2002) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(02)00294-x · Cited by 419
Polycation gene delivery systems: escape from endosomes to cytosol
Cho, Y. W., Kim, J.-D., & Park, K. (2003) · Journal of Pharmacy and Pharmacology · doi:10.1211/002235703765951311 · Cited by 370
Metadata
- Authors
- Blessing, T., Kursa, M., Holzhauser, R., Kircheis, R., & Wagner, E.
- Venue
- Bioconjugate Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2001-06-23
- Volume
- 12
- Issue
- 4
- Pages
- 529-537
- ISSN
- 1043-1802 (ISSN-L)
- 1520-4812
- OpenAlex
- W1978828567
- Cited by
- 245
- References
- 1
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- closed
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