The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Interaction of the Nuclear GTP-Binding Protein Ran with Its Regulatory Proteins RCC1 and RanGAP1
Abstract
The guanine nucleotide dissociation and GTPase reactions of Ran, a Ras-related nuclear protein, have been investigated using different fluorescence techniques to determine how these reactions are stimulated by the guanine nucleotide exchange factor RCC1 and the other regulatory protein, RanGAP1 (GTPase-activating protein). The intrinsic GTPase of Ran is one-tenth of the rate of p21ras and is even lower in the Ran(Q69L) mutant. Under saturating conditions the rate constant for the RanGAP1 stimulated GTPase reaction is 2.1 s-1 at 25 degrees C, which is a 10(5)-fold stimulation, whereas RanGAP1 has no effect on Ran(Q69L). The intrinsic guanine nucleotide dissociation rates of Ran are also very low and are likewise increased 10(5)-fold by the exchange factor RCC1. Methods to describe the reaction kinetically are presented. The Ran(T24N) mutant, which is analogous to the S17N mutant of p21ras, has decreased relative affinities for both GDP/GTP and favors GDP binding. However, it was found to interact almost normally with RCC1. The combination of these properties leads to stabilization of the Ran(T24N)-RCC1 complex and may result in vivo in depletion of RCC1 available for stimulating guanine nucleotide exchange.
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Cites
The GTPase superfamily: conserved structure and molecular mechanism
Bourne, H. R., Sanders, D. A., & McCormick, F. (1991) · Nature · doi:10.1038/349117a0 · Cited by 3,392
A Cytoplasmic Protein Stimulates Normal N- ras p21 GTPase, But Does Not Affect Oncogenic Mutants
Trahey, M., & McCormick, F. (1987) · Science · doi:10.1126/science.2821624 · Cited by 1,211
Kinetics of interaction of nucleotides with nucleotide-free H-ras p21
John, J., Sohmen, R., Feuerstein, J., Linke, R., Wittinghofer, A., & Goody, R. S. (1990) · Biochemistry · doi:10.1021/bi00477a025 · Cited by 429
Self-citation
Cited by
Fagotto, F., Glück, U., & Gumbiner, B. M. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70082-x · Cited by 434
Silljé, H. H. W., Nagel, S., Körner, R., & Nigg, E. A. (2006) · Current Biology · doi:10.1016/j.cub.2006.02.070 · Cited by 267
Rossman, K. L., Worthylake, D. K., Snyder, J. T., Siderovski, D. P., Campbell, S. L., & Sondek, J. (2002) · The EMBO Journal · doi:10.1093/emboj/21.6.1315 · Cited by 222
The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP
Askjaer, P., Jensen, T. H., Nilsson, J., Englmeier, L., & Kjems, J. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.50.33414 · Cited by 219
Sculpting Organs: Mechanical Regulation of Tissue Development
Nelson, C. M., & Gleghorn, J. P. (2012) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071811-150043 · Cited by 130
Metadata
- Authors
- Klebe, C., Bischoff, F. R., Ponstingl, H., & Wittinghofer, A.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1995-01-01
- Volume
- 34
- Issue
- 2
- Pages
- 639-647
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2053569747
- Cited by
- 324
- References
- 3
- FWCI
- 6.16
- Type
- journal article
- Language
- en
- Open access
- closed
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- Klebe, C., Bischoff, F. R., Ponstingl, H., & Wittinghofer, A. (1995). Interaction of the Nuclear GTP-Binding Protein Ran with Its Regulatory Proteins RCC1 and RanGAP1. Biochemistry. https://doi.org/10.1021/bi00002a031
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