The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Fluorescence method for measuring the kinetics of fusion between biological membranes
Abstract
An assay is presented that allows continuous and sensitive monitoring of membrane fusion in both artificial and biological membrane systems. The method relies upon the relief of fluorescence self-quenching of octadecyl Rhodamine B chloride. When the probe is incorporated into a lipid bilayer at concentrations up to 9 mol% with respect to total lipid, the efficiency of self-quenching is proportional to its surface density. Upon fusion between membranes labeled with the probe and nonlabeled membranes, the decrease in surface density of the fluorophore results in a concomitant, proportional increase in fluorescence intensity, allowing kinetic and quantitative measurements of the fusion process. The kinetics of fusion between phospholipid vesicles monitored with this assay were found to be the same as those determined with a fusion assay based on resonance energy transfer [Struck, D. K., Hoekstra, D., & Pagano, R. E. (1981) Biochemistry 20, 4093-4099]. Octadecyl Rhodamine B chloride can be readily inserted into native biological membranes by addition of an ethanolic solution of the probe. Evidence is presented showing that the dilution of the fluorophore, occurring when octadecyl Rhodamine containing influenza virus is mixed with phospholipid vesicles at pH 5.0, but not pH 7.4, resulted from virus-vesicle fusion and was not related to processes other than fusion. Furthermore, by use of this method, the kinetics of fusion between Sendai virus and erythrocyte ghosts and virus-induced fusion of ghosts were readily revealed. Dilution of the probe was not observed upon prior treatment of fluorescently labeled Sendai virus with trypsin.(ABSTRACT TRUNCATED AT 250 WORDS)
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Citations
Cites
Endocytosis and the recycling of plasma membrane.
Steinman, R. M., Mellman, I. S., Muller, W. A., & Cohn, Z. A. (1983) · The Journal of cell biology · doi:10.1083/jcb.96.1.1 · Cited by 1,450
Use of resonance energy transfer to monitor membrane fusion
Struck, D. K., Hoekstra, D., & Pagano, R. E. (1981) · Biochemistry · doi:10.1021/bi00517a023 · Cited by 1,277
Self-citation[16] Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes
Steck, T. L., & Kant, J. A. (1974) · Methods in Enzymology · doi:10.1016/0076-6879(74)31019-1 · Cited by 1,014
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Membrane fusion proteins of enveloped animal viruses
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Self-citationpH-sensitive liposomes: acid-induced liposome fusion.
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Self-citationAggregation and fusion of phospholipid vesicles
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Self-citationMaeda, T., Kawasaki, K., & Ohnishi, S. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.7.4133 · Cited by 175
Papahadjopoulos, D., & Kimelberg, H. K. (1974) · Progress in Surface Science · doi:10.1016/s0079-6816(74)80006-7 · Cited by 161
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Modulation of membrane fusion by ionotropic and thermotropic phase transitions
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Cited by
Ultee, A., Bennik, M. H. J., & Moezelaar, R. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.4.1561-1568.2002 · Cited by 1,602
Qiao, L., Hu, S., Huang, K., Su, T., Li, Z., Vandergriff, A., Cores, J., Dinh, P.-U., Allen, T., Shen, D., et al. (2020) · Theranostics · doi:10.7150/thno.39434 · Cited by 404
Imaging extracellular vesicles: current and emerging methods
Chuo, S. T.-Y., Chien, J. C.-Y., & Lai, C. P.-K. (2018) · Journal of Biomedical Science · doi:10.1186/s12929-018-0494-5 · Cited by 359
Glaser, P. E., & Gross, R. W. (1995) · Biochemistry · doi:10.1021/bi00038a013 · Cited by 210
Han, X., Ramanadham, S., Turk, J., & Gross, R. W. (1998) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(98)00154-0 · Cited by 31
Metadata
- Authors
- Hoekstra, D., De Boer, T., Klappe, K., & Wilschut, J.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1984-11-01
- Volume
- 23
- Issue
- 24
- Pages
- 5675-5681
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2074685112
- Cited by
- 677
- References
- 18
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- closed
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