Cited by 50DNA Repair MechanismsDNA and Nucleic Acid ChemistryBiochemical and Molecular Research

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Hydrolysis of 3'-terminal mispairs in vitro by the 3' .fwdarw. 5' exonuclease of DNA polymerase .delta. permits subsequent extension by DNA polymerase .alpha.

Perrino, Fred W., Loeb, Lawrence A.·1990·Biochemistry

Open this paper in CoChat — free account

Abstract

Purified DNA polymerase alpha, the major replicating enzyme found in mammalian cells, lacks an associated 3'----5' proofreading exonuclease that, in bacteria, contributes significantly to the accuracy of DNA replication. Calf thymus DNA polymerase alpha cannot remove mispaired 3'-termini, nor can it extend them efficiently. We designed a biochemical assay to search in cell extracts for a putative proofreading exonuclease that might function in concert with DNA polymerase alpha in vivo but dissociates from it during purification. Using this assay, we purified a 3'----5' exonuclease from calf thymus that preferentially hydrolyzes mispaired 3'-termini, permitting subsequent extension of the correctly paired 3'-terminus by DNA polymerase alpha. This exonuclease copurifies with a DNA polymerase activity that is biochemically distinct from DNA polymerase alpha and exhibits characteristics described for a second replicative DNA polymerase, DNA polymerase delta. In related studies, we showed that the 3'----5' exonuclease of authentic DNA polymerase delta, like the purified exonuclease, removes terminal mispairs, allowing extension by DNA polymerase alpha. These data suggest that a single proofreading exonuclease could be shared by DNA polymerases alpha and delta, functioning at the site of DNA replication in mammalian cells.

Integrity signals

Registry

Crossref · 10.1021/bi00474a002

Cites

30 of 30 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Enzymatic Synthesis of Deoxyribonucleic Acid
Brutlag, D. L., & Kornberg, A. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45781-5 · Cited by 599
An auxiliary protein for DNA polymerase-delta from fetal calf thymus.
Tan, C. K., Castillo, C., So, A. G., & Downey, K. M. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67240-0 · Cited by 496
Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases.
Wong, S. W., Wahl, A. F., Yuan, P.-M., Arai, N., Pearson, B. E., Arai, K., Korn, D., Hunkapiller, M. W., & Wang, T. S. ‐. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02781.x · Cited by 404
Coordinated leading and lagging strand synthesis during SV40 DNA replication in vitro requires PCNA
Prelich, G., & Stillman, B. (1988) · Cell · doi:10.1016/0092-8674(88)90493-x · Cited by 392
A separate editing exonuclease for DNA replication: the epsilon subunit of Escherichia coli DNA polymerase III holoenzyme.
Scheuermann, R. H., & Echols, H. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.24.7747 · Cited by 253
Further studies on calf thymus DNA polymerase .delta. purified to homogeneity by a new procedure
Lee, M., Tan, C. K., Downey, K. M., & So, A. G. (1984) · Biochemistry · doi:10.1021/bi00304a003 · Cited by 205
Human DNA polymerase α: predicted functional domains and relationships with viral DNA polymerases
Wang, T. S., Wong, S. W., & Korn, D. A. (1989) · The FASEB Journal · doi:10.1096/fasebj.3.1.2642867 · Cited by 203
DNA Polymerase III Holoenzyme Of Escherichia Coli
McHenry, C. S. (1988) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.57.1.519 · Cited by 186
DNA polymerase α
Lehman, I., & Kaguni, L. S. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83733-4 · Cited by 161
DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha.
Wang, T. S., Hu, S., & Korn, D. A. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43487-9 · Cited by 158

Show all 30 references

DNA Replication Fidelity
Kunkel, T. A., & Bebenek, K. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.497 · Cited by 1,003
DNA Replication Fidelity
Kunkel, T. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.r400006200 · Cited by 754
Eukaryotic Mismatch Repair in Relation to DNA Replication
Kunkel, T. A., & Erie, D. A. (2015) · Annual Review of Genetics · doi:10.1146/annurev-genet-112414-054722 · Cited by 459
The 3′–5′ exonucleases
Shevelev, I. V., & Hübscher, U. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm804 · Cited by 292
Identification and Expression of the TREX1 and TREX2 cDNA Sequences Encoding Mammalian 3′→5′ Exonucleases
Mazur, D. J., & Perrino, F. W. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.28.19655 · Cited by 272
Self-citation
Shieldin – the protector of DNA ends
Setiaputra, D., & Durocher, D. (2019) · EMBO Reports · doi:10.15252/embr.201847560 · Cited by 230
The 3′ → 5′ exonucleases of both DNA polymerases δ and ε participate in correcting errors of DNA replication in Saccharomyces cerevisiae
Morrison, A., & Sugino, A. (1994) · Molecular and General Genetics MGG · doi:10.1007/bf00280418 · Cited by 205
Evidence that Errors Made by DNA Polymerase α are Corrected by DNA Polymerase δ
Pavlov, Y. I., Frahm, C., McElhinny, S. A. N., Niimi, A., Suzuki, M., & Kunkel, T. A. (2006) · Current Biology · doi:10.1016/j.cub.2005.12.002 · Cited by 177
Genomic Instability and Tumor Progression: Mechanistic Considerations
Cheng, K. C., & Loeb, L. A. (1992) · Advances in cancer research · doi:10.1016/s0065-230x(08)60824-6 · Cited by 173
Self-citation
Defective DNA polymerase-δ proofreading causes cancer susceptibility in mice
Goldsby, R. E., Lawrence, N. A., Hays, L. E., Olmsted, E. A., Chen, X., Singh, M., & Preston, B. D. (2001) · Nature Medicine · doi:10.1038/88963 · Cited by 171
High incidence of epithelial cancers in mice deficient for DNA polymerase δ proofreading
Goldsby, R. E., Hays, L. E., Chen, X., Olmsted, E. A., Slayton, W. B., Spangrude, G. J., & Preston, B. D. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.232340999 · Cited by 166
DNA Polymerases Divide the Labor of Genome Replication
Lujan, S. A., Williams, J. S., & Kunkel, T. A. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2016.04.012 · Cited by 156
DNA polymerase delta in dna replication and genome maintenance
Prindle, M. J., & Loeb, L. A. (2012) · Environmental and Molecular Mutagenesis · doi:10.1002/em.21745 · Cited by 139
Self-citation
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins
Zhong, X., Garg, P., Stith, C. M. W., McElhinny, S. A. N., Kissling, G. E., Burgers, P., & Kunkel, T. A. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl465 · Cited by 124
In Vivo Consequences of Putative Active Site Mutations in Yeast DNA Polymerases α, ε, δ, and ζ
Pavlov, Y. I., Shcherbakova, P. V., & Kunkel, T. A. (2001) · Genetics · doi:10.1093/genetics/159.1.47 · Cited by 122
Properties of DNA polymerases δ and ϵ, and their roles in eukaryotic DNA replication
Bambara, R. A., & Jessee, C. B. (1991) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(91)90147-e · Cited by 116
Mutator phenotypes due to DNA replication infidelity
Arana, M. E., & Kunkel, T. A. (2010) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2010.10.003 · Cited by 103
High fidelity and lesion bypass capability of human DNA polymerase δ
Schmitt, M. W., Matsumoto, Y., & Loeb, L. A. (2009) · Biochimie · doi:10.1016/j.biochi.2009.06.007 · Cited by 92
Self-citation
Enzymatic switching for efficient and accurate translesion DNA replication.
McCulloch, S. D., Kokoska, R., Chilkova, O., Welch, C. M., Johansson, E., Burgers, P., & Kunkel, T. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh777 · Cited by 97
Evidence from mutational specificity studies that yeast DNA polymerases δ and ϵ replicate different DNA strands at an intracellular replication fork 1 1Edited by A. R. Fersht
Karthikeyan, R., Vonarx, E. J., Straffon, A. F. L., Simon, M., Faye, G., & Kunz, B. A. (2000) · Journal of Molecular Biology · doi:10.1006/jmbi.2000.3744 · Cited by 76
Eukaryotic DNA Replication
So, A. G., & Downey, K. M. (1992) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239209082561 · Cited by 75
Effects of Escherichia coli dnaE antimutator alleles in a proofreading-deficient mutD5 strain
Fijałkowska, I. J., & Schaaper, R. M. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.20.5979-5986.1995 · Cited by 70
Connections between translation, transcription and replication error-rates
Ninio, J. (1991) · Biochimie · doi:10.1016/0300-9084(91)90186-5 · Cited by 61
Exonucleolytic proofreading of leading and lagging strand DNA replication errors.
Roberts, J. D., Thomas, D. C., & Kunkel, T. A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.8.3465 · Cited by 57
Quantifying the contributions of base selectivity, proofreading and mismatch repair to nuclear DNA replication in Saccharomyces cerevisiae
Charles, J. A. S., Liberti, S. E., Williams, J. S., Lujan, S. A., & Kunkel, T. A. (2015) · DNA repair · doi:10.1016/j.dnarep.2015.04.006 · Cited by 61
Incorporation of cytosine arabinoside monophosphate into DNA at internucleotide linkages by human DNA polymerase alpha
Perrino, F. W., & Mekosh, H. L. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50053-3 · Cited by 45
Self-citation
Exonucleolytic proofreading by p53 protein
Bakhanashvili, M. (2001) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2001.02075.x · Cited by 47
The role of DNA exonucleases in protecting genome stability and their impact on ageing
Mason, P. A., & Cox, L. S. (2011) · AGE · doi:10.1007/s11357-011-9306-5 · Cited by 46
Selective action of 4'-azidothymidine triphosphate on reverse transcriptase of human immunodeficiency virus type 1 and human DNA polymerases .alpha. and .beta.
Chen, M. S., Suttmann, R. T., Papp, E., Cannon, P. D., McRoberts, M. J., Bach, C., Copeland, W. C., & Wang, T. S. ‐. (1993) · Biochemistry · doi:10.1021/bi00074a011 · Cited by 44
Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations
Pavlov, Y. I., Maki, S., Maki, H., & Kunkel, T. A. (2004) · BMC Biology · doi:10.1186/1741-7007-2-11 · Cited by 41
Animal cell DNA polymerases in DNA repair
Perrino, F. W., & Loeb, L. A. (1990) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(90)90012-t · Cited by 41
Self-citation
DNA polymerase δ proofreads errors made by DNA polymerase ε
Bulock, C. R., Xing, X., & Shcherbakova, P. V. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1917624117 · Cited by 30
p53 enhances the fidelity of DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase
Bakhanashvili, M. (2001) · Oncogene · doi:10.1038/sj.onc.1204956 · Cited by 30
Loss of base excision repair in aging rat neurons and its restoration by DNA polymerase β
Rao, K. S., Annapurna, V., Raji, N., & Harikrishna, T. (2000) · Molecular Brain Research · doi:10.1016/s0169-328x(00)00266-7 · Cited by 29
Cleavage of Substrates with Mismatched Nucleotides by Flap Endonuclease-1
Rumbaugh, J. A., Henricksen, L. A., DeMott, M. S., & Bambara, R. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.21.14602 · Cited by 27
Inhibition of the 3′→ 5′ Exonuclease of Human DNA Polymerase ε by Fludarabine-terminated DNA
Kamiya, K.-I., Huang, P., & Plunkett, W. K. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.32.19428 · Cited by 27
How asymmetric DNA replication achieves symmetrical fidelity
Zhou, Z.-X., Lujan, S. A., Burkholder, A., Charles, J. S., Dahl, J. M., Farrell, C. E., Williams, J. S., & Kunkel, T. A. (2021) · Nature Structural & Molecular Biology · doi:10.1038/s41594-021-00691-6 · Cited by 26
Antimutator variants of DNA polymerases
Herr, A. J., Williams, L. N., & Preston, B. D. (2011) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2011.620941 · Cited by 24
An error-correcting proofreading exonuclease-polymerase that copurifies with DNA-polymerase-alpha-primase.
Bialek, G., & Große, F. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53421-9 · Cited by 22
DNA Replication Fidelity: Proofreading in Trans
Albertson, T., & Preston, B. D. (2006) · Current Biology · doi:10.1016/j.cub.2006.02.031 · Cited by 23
Delta-type DNA polymerase characterized fromDrosophila melanogasterembryos
Peck, V. M., Gerner, E. W., & Cress, A. E. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.21.5779 · Cited by 15
Search for DNA repair pathways in Drosophila melanogaster
de Buendı́a, P. G. (1998) · Mutation Research/DNA Repair · doi:10.1016/s0921-8777(97)00063-3 · Cited by 13
A nuclear 3′–5′ exonuclease proofreads for the exonuclease-deficient DNA polymerase α
Brown, K. R., Weatherdon, K. L., Galligan, C. L., & Skalski, V. (2002) · DNA repair · doi:10.1016/s1568-7864(02)00115-5 · Cited by 11
Family A and B DNA Polymerases in Cancer: Opportunities for Therapeutic Interventions
Shanbhag, V., Sachdev, S., Flores, J., Modak, M. J., & Singh, K. (2018) · Biology · doi:10.3390/biology7010005 · Cited by 9
Extrinsic proofreading
Zhou, Z.-X., & Kunkel, T. A. (2022) · DNA repair · doi:10.1016/j.dnarep.2022.103369 · Cited by 8
Opportunities for new studies of nuclear DNA replication enzymology in budding yeast
Garbacz, M. A., Lujan, S. A., & Kunkel, T. A. (2019) · Current Genetics · doi:10.1007/s00294-019-01023-4 · Cited by 4
Molecular Concepts of Macrophage Targeting
Boldescu, V., Crudu, V., Sucman, N. S., Pogrebnoi, S. I., Zviaghinţeva, M., Stingaci, E., Pogrebnoi, V., & Macaev, F. (2013) · Chemistry Journal of Moldova · doi:10.19261/cjm.2013.08(2).02 · Cited by 3
The Role of P53 Exonuclease in Accuracy of DNA Synthesis and Sensitivity to Nucleoside Analogs in Various Compartments of Cells
Rahav, G., & Bakhanashvili, M. (2013) · InTech eBooks · doi:10.5772/54220
IDENTIFICATION OF ACTIVITIES INVOLVED IN CAG/CTG REPEAT INSTABILITY
Chan, N. L. S. (2011) · UKnowledge (University of Kentucky)
Gene Expression of Lipid Binding Protein Transferred the Ability of Specific Attachment of Hemopoietic Cells to Non-supportive Stromal Cell Line, MS-K.
Takashita, E., Sugimoto, K., Jiang, H., Tohma, M., Urano, E., Yokoyama, H., & Mori, K. J. (1997) · Cell Structure and Function · doi:10.1247/csf.22.595

Authors: Perrino, F. W., & Loeb, L. A.
Affiliations: University of Washington (US)
ORCID: Perrino, Fred W.
Venue: Biochemistry
Publisher: American Chemical Society (ACS)
Published: 1990-06-05
Volume: 29
Issue: 22
Pages: 5226-5231
ISSN: 0006-2960 (ISSN-L)
1520-4995
DOI: 10.1021/bi00474a002
OpenAlex: W2058420479
Cited by: 50
References: 30
Citations by year: 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 1 · 2015: 2 · 2013: 2 · 2012: 1
FWCI: 1.43
Type: journal article
Language: en
Open access: closed
Locations: Biochemistry (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r35ca039903-14)
National Cancer Institute (T32 CA-09437, T32CA009437, R 35-CA-39903)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords: DNA polymerase delta, 3'-5' exonuclease, proofreading exonuclease, DNA polymerase alpha, DNA replication fidelity, calf thymus
MeSH terms: Animals, Base Sequence, Cattle, DNA Polymerase II, DNA Polymerase III, DNA-Directed DNA Polymerase, Exonucleases, Genetic Techniques, Thymus Gland

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.

Hydrolysis of 3'-terminal mispairs in vitro by the 3' .fwdarw. 5' exonuclease of DNA polymerase .delta. permits subsequent extension by DNA polymerase .alpha.

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks

Data sources