The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Hydrolysis of 3'-terminal mispairs in vitro by the 3' .fwdarw. 5' exonuclease of DNA polymerase .delta. permits subsequent extension by DNA polymerase .alpha.
Abstract
Purified DNA polymerase alpha, the major replicating enzyme found in mammalian cells, lacks an associated 3'----5' proofreading exonuclease that, in bacteria, contributes significantly to the accuracy of DNA replication. Calf thymus DNA polymerase alpha cannot remove mispaired 3'-termini, nor can it extend them efficiently. We designed a biochemical assay to search in cell extracts for a putative proofreading exonuclease that might function in concert with DNA polymerase alpha in vivo but dissociates from it during purification. Using this assay, we purified a 3'----5' exonuclease from calf thymus that preferentially hydrolyzes mispaired 3'-termini, permitting subsequent extension of the correctly paired 3'-terminus by DNA polymerase alpha. This exonuclease copurifies with a DNA polymerase activity that is biochemically distinct from DNA polymerase alpha and exhibits characteristics described for a second replicative DNA polymerase, DNA polymerase delta. In related studies, we showed that the 3'----5' exonuclease of authentic DNA polymerase delta, like the purified exonuclease, removes terminal mispairs, allowing extension by DNA polymerase alpha. These data suggest that a single proofreading exonuclease could be shared by DNA polymerases alpha and delta, functioning at the site of DNA replication in mammalian cells.
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“For example, extrinsic exonucleolytic proofreading could improve the fidelity of Pol ␣ (51) during initiation of Okazaki fragments, the fidelity of Pol at a replication fork (52), or the fidelity of Pol  during single-nucleotide BER (53).”
From DNA Replication Fidelity (2004) · cites this paper as [51]
Citations
Cites
Enzymatic Synthesis of Deoxyribonucleic Acid
Brutlag, D. L., & Kornberg, A. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45781-5 · Cited by 599
An auxiliary protein for DNA polymerase-delta from fetal calf thymus.
Tan, C. K., Castillo, C., So, A. G., & Downey, K. M. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67240-0 · Cited by 496
Wong, S. W., Wahl, A. F., Yuan, P.-M., Arai, N., Pearson, B. E., Arai, K., Korn, D., Hunkapiller, M. W., & Wang, T. S. ‐. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02781.x · Cited by 404
Coordinated leading and lagging strand synthesis during SV40 DNA replication in vitro requires PCNA
Prelich, G., & Stillman, B. (1988) · Cell · doi:10.1016/0092-8674(88)90493-x · Cited by 392
Scheuermann, R. H., & Echols, H. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.24.7747 · Cited by 253
Further studies on calf thymus DNA polymerase .delta. purified to homogeneity by a new procedure
Lee, M., Tan, C. K., Downey, K. M., & So, A. G. (1984) · Biochemistry · doi:10.1021/bi00304a003 · Cited by 205
Human DNA polymerase α: predicted functional domains and relationships with viral DNA polymerases
Wang, T. S., Wong, S. W., & Korn, D. A. (1989) · The FASEB Journal · doi:10.1096/fasebj.3.1.2642867 · Cited by 203
DNA Polymerase III Holoenzyme Of Escherichia Coli
McHenry, C. S. (1988) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.57.1.519 · Cited by 186
Lehman, I., & Kaguni, L. S. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83733-4 · Cited by 161
Wang, T. S., Hu, S., & Korn, D. A. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43487-9 · Cited by 158
Cited by
Kunkel, T. A., & Bebenek, K. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.497 · Cited by 1,003
Kunkel, T. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.r400006200 · Cited by 754
Eukaryotic Mismatch Repair in Relation to DNA Replication
Kunkel, T. A., & Erie, D. A. (2015) · Annual Review of Genetics · doi:10.1146/annurev-genet-112414-054722 · Cited by 459
Shevelev, I. V., & Hübscher, U. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm804 · Cited by 292
Mazur, D. J., & Perrino, F. W. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.28.19655 · Cited by 272
Self-citation
Metadata
- Authors
- Perrino, F. W., & Loeb, L. A.
- Affiliations
- University of Washington (US)
- ORCID
- Perrino, Fred W.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1990-06-05
- Volume
- 29
- Issue
- 22
- Pages
- 5226-5231
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2058420479
- Cited by
- 50
- References
- 30
- Citations by year
- 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2016: 1 · 2015: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r35ca039903-14)
- National Cancer Institute (T32 CA-09437, T32CA009437, R 35-CA-39903)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- DNA polymerase delta, 3'-5' exonuclease, proofreading exonuclease, DNA polymerase alpha, DNA replication fidelity, calf thymus
- MeSH terms
- Animals, Base Sequence, Cattle, DNA Polymerase II, DNA Polymerase III, DNA-Directed DNA Polymerase, Exonucleases, Genetic Techniques, Thymus Gland
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