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Antigenicity of elastin: characterization of major antigenic determinants on purified insoluble elastin
Abstract
This investigation reports a systematic comparison of antibody responses to well-defined fragments of mature, insoluble elastin in order to identify antigenic regions of the molecule. Antiserum to insoluble elastin was used to screen by radioimmunoassay the serologic activity of elastin peptides purified from a thermolysin digestion of insoluble elastin. Fractions with positive antigenicity were characterized by amino acid analysis and protein sequence analysis. Our results indicate that antigenic determinants on elastin can be classified functionally into two categories: major determinants related to the cross-linking domain which show cross-reactivity between species and limited, species-specific determinants in non-cross-linked regions of the molecule.
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“Elastin denaturation during the decellularization may also produce elastin particles with significant antigenicity [115].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [115]
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Metadata
- Authors
- Mecham, R. P., & Lange, G.
- Affiliations
- Washington University in St. Louis (US)
- ORCID
- Mecham, Robert P.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1982-02-16
- Volume
- 21
- Issue
- 4
- Pages
- 669-673
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2007829335
- Cited by
- 57
- References
- 15
- Citations by year
- 2025: 1 · 2023: 4 · 2017: 2 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Connective tissue disorders research
- Keywords
- elastin, antigenic determinants, antibody response, cross-species reactivity, species-specific epitopes, thermolysin digestion, elastin peptides, epitope mapping
- MeSH terms
- Animals, Antibody Specificity, Epitopes, Cattle, Elastin, Peptide Fragments, Protein Conformation, Rabbits, Species Specificity
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