The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Nonspecific interaction of lac repressor with DNA: an association reaction driven by counterion release
Abstract
We have investigated the nonspecific interaction of lac repressor protein with DNA by a quantitative application of DNA-cellulose chromatography (deHaseth, P.L., et al. (1977), Biochemistry 16 (third of five papers in a series in this issue)). The observed association constant for the interaction, KRD obsd, is a sensitive function of ion concentrations and pH. Application of binding theory to interpret these effects gives the results that 11 +/- 2 monovalent ions are released in the interaction and two groups on repressor must be protonated for repressor to bind to DNA. We argue that much of the ion release results from the displacement of cations from the DNA, and estimate on this basis that 12 +/- 2 phosphates are involved in ionic interactions with the protein. Ion release drives the protonation reaction and the overall repressor-DNA interaction. The major role of low molecular weight ions in the repressor-DNA interaction suggests that ion concentration changes must be considered in discussing mechanisms of control of gene expression.
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Lin, S.-Y., & Riggs, A. D. (1975) · Cell · doi:10.1016/0092-8674(75)90116-6 · Cited by 338
Cited by
Interactions of bacteriophage T4-coded gene 32 protein with nucleic acids
Kowalczykowski, S. C., Lonberg, N., Newport, J. W., & von Hippel, P. H. (1981) · Journal of Molecular Biology · doi:10.1016/0022-2836(81)90335-1 · Cited by 339
Martin, E. W., & Holehouse, A. S. (2020) · Emerging Topics in Life Sciences · doi:10.1042/etls20190164 · Cited by 281
Richey, B., Cayley, D. S., Mossing, M. C., Kolka, C., Anderson, C. F., Farrar, T. C., & Record, M. T. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48218-x · Cited by 270
Self-citationFrom Water and Ions to Crowded Biomacromolecules: In Vivo Structuring of a Prokaryotic Cell
Spitzer, J. (2011) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00010-11 · Cited by 77
Metadata
- Authors
- DeHaseth, P. L., Lohman, T. M., & Record, M. T.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1977-11-01
- Volume
- 16
- Issue
- 22
- Pages
- 4783-4790
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2090759101
- Cited by
- 262
- References
- 1
- FWCI
- 3.88
- Type
- journal article
- Language
- en
- Open access
- closed
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- DeHaseth, P. L., Lohman, T. M., & Record, M. T. (1977). Nonspecific interaction of lac repressor with DNA: an association reaction driven by counterion release. Biochemistry. https://doi.org/10.1021/bi00641a004
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
