Nitrite Reductase Activity of Nonsymbiotic Hemoglobins from Arabidopsis thaliana
Open accessAbstract
Plant nonsymbiotic hemoglobins possess hexacoordinate heme geometry similar to that of the heme protein neuroglobin. We recently discovered that deoxygenated neuroglobin converts nitrite to nitric oxide (NO), an important signaling molecule involved in many processes in plants. We sought to determine whether Arabidopsis thaliana nonsymbiotic hemoglobins classes 1 and 2 (AHb1 and AHb2, respectively) might function as nitrite reductases. We found that the reaction of nitrite with deoxygenated AHb1 and AHb2 generates NO gas and iron-nitrosyl-hemoglobin species. The bimolecular rate constants for reduction of nitrite to NO are 19.8 ± 3.2 and 4.9 ± 0.2 M(-1) s(-1), respectively, at pH 7.4 and 25 °C. We determined the pH dependence of these bimolecular rate constants and found a linear correlation with the concentration of protons, indicating the requirement for one proton in the reaction. The release of free NO gas during the reaction under anoxic and hypoxic (2% oxygen) conditions was confirmed by chemiluminescence detection. These results demonstrate that deoxygenated AHb1 and AHb2 reduce nitrite to form NO via a mechanism analogous to that observed for hemoglobin, myoglobin, and neuroglobin. Our findings suggest that during severe hypoxia and in the anaerobic plant roots, especially in species submerged in water, nonsymbiotic hemoglobins provide a viable pathway for NO generation via nitrite reduction.
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When Ca2+ is bound, the EF hands fold over the reductase CaM element to relieve autoinhibition.
“When Ca2+ is bound, the EF hands fold over the reductase CaM element to relieve autoinhibition (63, 944).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [944]
The reductase domain of NOX5 includes a CaM-binding motif not found in the NOX1-4 proteins.
“The reductase domain of NOX5 also includes a CaM-binding motif not found in the NOX1–4 proteins.”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [944]
NOX5 has a similar architecture to NOX1-4 but has an additional N-terminal domain before the transmembrane domain with four EF hand motifs that are responsive to Ca2+.
“NOX5 has a similar architecture but has an additional N-terminal domain before the transmembrane domain with four EF hand motifs that are responsive to Ca2+.”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [944]
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- Authors
- Tiso, M., Tejero, J., Kenney, C., Frizzell, S., & Gladwin, M. T.
- Affiliations
- National Institutes of Health (US)
- University of Pittsburgh (US)
- Corresponding author
- Tiso, Mauro
- Gladwin, Mark T.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-06-20
- Volume
- 51
- Issue
- 26
- Pages
- 5285-5292
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- PMC
- PMC3857030
- OpenAlex
- W1979664345
- Cited by
- 69
- References
- 51
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 5 · 2021: 4 · 2020: 7 · 2019: 2 · 2018: 5 · 2017: 4 · 2016: 7 · 2015: 7 · 2014: 6 · 2013: 11 · 2012: 2
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R01HL096973, P01HL103455, R01HL098032)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heme Oxygenase-1 and Carbon Monoxide
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Keywords
- nitrite reductase activity, nitric oxide production, Arabidopsis thaliana, neuroglobin, pH dependence, bimolecular rate constants, hypoxia, submerged plants, chemiluminescence
- MeSH terms
- Hemoglobins, Hydrogen-Ion Concentration, Nitric Oxide, Nitrite Reductases, Nitrites, Arabidopsis, Arabidopsis Proteins
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