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Detection of Antigen−Antibody Binding Events with the Atomic Force Microscope
Abstract
An atomic force microscope (AFM) has been used to directly monitor specific interactions between antibodies and antigens employed in an immunoassay system. Results were achieved using AFM probes functionalized with ferritin, and monitoring the adhesive forces between the probe and anti-ferritin antibody-coated substrates. Analysis of the force distribution data suggests a quantization of the forces, with a period of 49 +/- 10 pN. This periodic force may be attributed to single unbinding events between individual antigen and antibody molecules. These results demonstrate that the AFM could be employed as an analytical tool to study the interactions between the molecules involved in biosensor systems. The potential of the technique to provide information relating to the manner in which the antibody molecule binds to its specific antigen is also discussed.
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Citations
Cited by
The effect of sedimentation and diffusion on cellular uptake of gold nanoparticles
Cho, E. C., Zhang, Q., & Xia, Y. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2011.58 · Cited by 719
AFM: a versatile tool in biophysics
Alessandrini, A., & Facci, P. (2005) · Measurement Science and Technology · doi:10.1088/0957-0233/16/6/r01 · Cited by 384
Single molecule force spectroscopy in biology using the atomic force microscope
Zlatanova, J., Lindsay, S. M., & Leuba, S. H. (2000) · Progress in Biophysics and Molecular Biology · doi:10.1016/s0079-6107(00)00014-6 · Cited by 375
Spatially resolved force spectroscopy of biological surfaces using the atomic force microscope
Heinz, W. F., & Hoh, J. H. (1999) · Trends in Biotechnology · doi:10.1016/s0167-7799(99)01304-9 · Cited by 373
Single Integrin Molecule Adhesion Forces in Intact Cells Measured by Atomic Force Microscopy
Lehenkari, P. P., & Horton, M. A. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0827 · Cited by 289
Metadata
- Authors
- Allen, S., Chen, X., Davies, J., Davies, M. C., Dawkes, A. C., Edwards, J. C., Roberts, C. J., Sefton, J., Tendler, S. J. B., & Williams, P. M.
- Venue
- Biochemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-06-01
- Volume
- 36
- Issue
- 24
- Pages
- 7457-7463
- ISSN
- 0006-2960 (ISSN-L)
- 1520-4995
- OpenAlex
- W2032641879
- Cited by
- 341
- References
- 0
- FWCI
- 14.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Allen, S., Chen, X., Davies, J., Davies, M. C., Dawkes, A. C., Edwards, J. C., Roberts, C. J., Sefton, J., Tendler, S. J. B., & Williams, P. M. (1997). Detection of Antigen−Antibody Binding Events with the Atomic Force Microscope. Biochemistry. https://doi.org/10.1021/bi962531z
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