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Cited by 240

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Nearest Neighbor Thermodynamic Parameters for Internal G·A Mismatches in DNA

Allawi, Hatim T., SantaLucia, John·1998·Biochemistry

Abstract

Thermodynamics of 22 oligonucleotides with internal single G.A mismatches dissolved in 1 M NaCl were determined from absorbance versus temperature melting curves. These data, combined with five literature sequences, were used to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal G.A mismatches and Watson-Crick flanking base pairs. The G.A mismatch parameters predict DeltaG degrees 37, DeltaH degrees, DeltaS degrees, and TM with average deviations of 4.4%, 7.4%, 8.0%, and 1.5 degrees C, respectively. The nearest-neighbor parameters show that G.A mismatch stability is strongly context dependent, and DeltaG degrees 37 ranges from +1.16 kcal/mol for TGA/AAT to -0.78 kcal/mol for GGC/CAG. In addition, one-dimensional 1H NMR spectra show that the G.A pairing geometry is pH and context dependent.

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Crossref · 10.1021/bi9724873

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Metadata

Authors
Allawi, H. T., & SantaLucia, J.
Venue
Biochemistry
Published
1998-02-01
Volume
37
Issue
8
Pages
2170-2179
ISSN
  • 0006-2960 (ISSN-L)
  • 1520-4995
OpenAlex
W2077433900
Cited by
240
References
2
FWCI
2.93
Type
journal article
Language
en
Open access
closed

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