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Cited by 677

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR

Open access
Marshall, Owen J.·2004·Bioinformatics

Abstract

Summary: PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer–dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design. Availability: Open-source and freely available from http://perlprimer.sourceforge.net

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Metadata

Authors
Marshall, O. J.
Venue
Bioinformatics
Published
2004-04-08
Volume
20
Issue
15
Pages
2471-2472
ISSN
  • 1367-4803 (ISSN-L)
  • 1367-4811
OpenAlex
W2128862717
Cited by
677
References
11
FWCI
1.97
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. https://app.cochat.ai/papers/10.1093/bioinformatics/bth254
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Marshall, O. J. (2004). PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics. https://doi.org/10.1093/bioinformatics/bth254

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.