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Optimization of Chitin Extraction from Shrimp Shells
Abstract
The aim of this paper is to define optimal conditions for the extraction of chitin from shrimp shells. The kinetics of both demineralization and deproteinization with, in the latter case, the role of temperature are studied. The characterization of the residual calcium and protein contents, the molecular weights, and degrees of acetylation (DA) allows us to propose the optimal conditions as follows. The demineralization is completely achieved within 15 min at ambient temperature in an excess of HCl 0.25 M (with a solid-to-liquid ratio of about 1/40 (w/v)). The deproteinization is conveniently obtained in NaOH 1 M within 24 h at a temperature close to 70 degrees C with no incidence on the molecular weight or the DA. In these conditions, the residual content of calcium in chitin is below 0.01%, and the DA is almost 95%.
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Cited by
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Thomas, P., Lai, C. W., & Bin Johan, M. R. (2019) · Journal of Analytical and Applied Pyrolysis · doi:10.1016/j.jaap.2019.03.021 · Cited by 207
Antonio, J. R., Antonio, C. R., Cardeal, I. L. S., Ballavenuto, J. M. A., & Oliveira, J. R. (2014) · Anais Brasileiros de Dermatologia · doi:10.1590/abd1806-4841.20142228 · Cited by 107
SEM characterization of anatomical variation in chitin organization in insect and arthropod cuticles
Chandran, R., Williams, L., Hung, A., Nowlin, K., & LaJeunesse, D. (2016) · Micron · doi:10.1016/j.micron.2015.12.010 · Cited by 89
Pohling, J., Hawboldt, K., & Dave, D. (2022) · Green Chemistry · doi:10.1039/d2gc01968j · Cited by 52
Metadata
- Authors
- Percot, A., Viton, C., & Domard, A.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-11-05
- Volume
- 4
- Issue
- 1
- Pages
- 12-18
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2014875517
- Cited by
- 470
- References
- 0
- FWCI
- 2.55
- Type
- journal article
- Language
- en
- Open access
- closed
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- Percot, A., Viton, C., & Domard, A. (2003). Optimization of Chitin Extraction from Shrimp Shells. Biomacromolecules. https://doi.org/10.1021/bm025602k
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