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Cited by 869

The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.

Protein Immobilization Strategies for Protein Biochips

Rusmini, Federica, Zhong, Zhiyuan, Feijen, Jan·2007·Biomacromolecules

Abstract

In the past few years, protein biochips have emerged as promising proteomic and diagnostic tools for obtaining information about protein functions and interactions. Important technological innovations have been made. However, considerable development is still required, especially regarding protein immobilization, in order to fully realize the potential of protein biochips. In fact, protein immobilization is the key to the success of microarray technology. Proteins need to be immobilized onto surfaces with high density in order to allow the usage of small amount of sample solution. Nonspecific protein adsorption needs to be avoided or at least minimized in order to improve detection performances. Moreover, full retention of protein conformation and activity is a challenging task to be accomplished. Although a large number of review papers on protein biochips have been published in recent years, few have focused on protein immobilization technology. In this review, current protein immobilization strategies, including physical, covalent, and bioaffinity immobilization for the fabrication of protein biochips, are described. Particular consideration has been given to oriented immobilization, also referred to as site-specific immobilization, which is believed will improve homogeneous surface covering and accessibility of the active site.

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Metadata

Authors
Rusmini, F., Zhong, Z., & Feijen, J.
Venue
Biomacromolecules
Published
2007-04-20
Volume
8
Issue
6
Pages
1775-1789
ISSN
  • 1525-7797 (ISSN-L)
  • 1526-4602
OpenAlex
W2032736963
Cited by
869
References
65
FWCI
17.36
Type
journal article
Language
en
Open access
closed

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